Chen Heng, Zheng Mengting, Li Mengyu, Zheng Yang, Wang Xu, He Yue
Department of Oral Maxillofacial & Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, PR China.
Department of Oral Maxillofacial & Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, PR China.
Free Radic Biol Med. 2024 Nov 20;225:805-820. doi: 10.1016/j.freeradbiomed.2024.10.308. Epub 2024 Oct 30.
Osteoradionecrosis of the jaw (ORNJ) is a severe complication following head and neck radiotherapy that significantly impacts the quality of life of patients. Currently, there is a lack of comprehensive understanding of the microenvironmental factors involved in ORNJ. In this study, we reveal the activation of taurine metabolism in irradiated mandibular stromal cells using scRNA-Seq and demonstrate a decrease in taurine levels in irradiated bone marrow mesenchymal stromal cells (BMSCs) through metabolomics. Compared with unirradiated BMSCs, taurine uptake in irradiated BMSCs increases. Taurine concentrations in the peripheral blood and jaws of irradiated mice are significantly lower than those in unirradiated mice (P = 0.0064 and 0.0249 respectively). Supplementation with taurine promotes osteogenic differentiation, reduces oxidative stress, and decreases DNA damage in irradiated BMSCs. Oral administration of taurine significantly improves the survival rate of irradiated mice and enhances osteogenesis in irradiated jaws. Our study highlights the role of taurine in the recovery from radiation-induced jaw injury, and suggests its potential as a non-invasive therapeutic option for combating ORNJ.
颌骨放射性骨坏死（ORNJ）是头颈部放疗后的一种严重并发症，会显著影响患者的生活质量。目前，人们对ORNJ所涉及的微环境因素缺乏全面了解。在本研究中，我们利用单细胞RNA测序揭示了照射后下颌基质细胞中牛磺酸代谢的激活，并通过代谢组学证明照射后骨髓间充质基质细胞（BMSC）中牛磺酸水平降低。与未照射的BMSC相比，照射后BMSC对牛磺酸的摄取增加。照射小鼠外周血和颌骨中的牛磺酸浓度显著低于未照射小鼠（分别为P = 0.0064和0.0249）。补充牛磺酸可促进照射后BMSC的成骨分化，降低氧化应激，并减少DNA损伤。口服牛磺酸可显著提高照射小鼠的存活率，并增强照射颌骨的成骨作用。我们的研究突出了牛磺酸在放射性颌骨损伤恢复中的作用，并表明其作为对抗ORNJ的非侵入性治疗选择的潜力。
