Chen Y, Zong C, Jia J, Liu Y, Zhang Z, Cai B, Tian L
State Key Laboratory of Military Stomatology and National Clinical Research Centre for Oral Diseases, and Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Xi'an, China; State Key Laboratory of Military Stomatology and National Clinical Research Centre for Oral Diseases, School of Stomatology, the Fourth Military Medical University, Xi'an, China.
State Key Laboratory of Military Stomatology and National Clinical Research Centre for Oral Diseases, School of Stomatology, the Fourth Military Medical University, Xi'an, China.
Int J Oral Maxillofac Surg. 2020 Dec;49(12):1648-1654. doi: 10.1016/j.ijom.2020.04.011. Epub 2020 May 23.
The aim of this study was to investigate the protective effect of hydrogen in a rat model of osteoradionecrosis of the jaw (ORNJ). The rats and bone marrow-derived mesenchymal stem cells (BMSCs) were pre-treated with hydrogen before receiving irradiation (7Gy per fraction, five fractions in total once a day for rats, 4Gy for BMSCs). Reactive oxygen species (ROS) and cell differentiation were measured in the BMSCs. Also, the radioprotective effect of hydrogen for ORNJ in Sprague-Dawley rats was examined by gross clinical manifestations, micro-computed tomography, and histology. Hydrogen significantly reduced the production of ROS in BMSCs after irradiation. The cell viability was significantly decreased after irradiation (P= 0.001), but pre-treatment with hydrogen before irradiation increased the cell viability (P= 0.025). Hydrogen considerably increased the cellular differentiation potential of the irradiated cells. Comparing with the rats underwent irradiaton only, those rats treated by hydrogen-rich saline significantly appeared improved occlusion, salivation, alopecia, oral ulcer, and less bone necrosis. Myofibroblasts accumulated overwhelmingly in the fibrosis medulla and around the sequestrum after irradiation, and this was decreased in the group pre-treated with hydrogen. Hydrogen may represent a strategy for the prevention and treatment of ORNJ. Its high efficacy and low toxicity suggest possible therapeutic application.
本研究的目的是在大鼠颌骨放射性骨坏死（ORNJ）模型中研究氢气的保护作用。在接受照射前（大鼠每天一次，每次7Gy，共5次；骨髓间充质干细胞（BMSCs）为4Gy），对大鼠和BMSCs进行氢气预处理。检测BMSCs中的活性氧（ROS）和细胞分化情况。此外，通过大体临床表现、微型计算机断层扫描和组织学检查氢气对Sprague-Dawley大鼠ORNJ的辐射防护作用。氢气显著降低了照射后BMSCs中ROS的产生。照射后细胞活力显著降低（P = 0.001），但照射前用氢气预处理可提高细胞活力（P = 0.025）。氢气显著增加了照射后细胞的分化潜能。与仅接受照射的大鼠相比，富氢盐水处理的大鼠在咬合、唾液分泌、脱发、口腔溃疡方面明显改善，骨坏死减少。照射后肌成纤维细胞大量积聚在纤维化髓质和死骨周围，而在氢气预处理组中这种情况有所减少。氢气可能是一种预防和治疗ORNJ的策略。其高效低毒提示了可能的治疗应用前景。
