The First Dongguan Affiliated Hospital, School of Pharmacy, Guangdong Medical University, Dongguan, 523808, China.
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Carbohydr Polym. 2025 Jan 1;347:122764. doi: 10.1016/j.carbpol.2024.122764. Epub 2024 Sep 17.
Emerging research attentions are focused on the development of fluorescent biomaterials for various biomedical applications, including fluorescence-guided surgery. However, it is still challenging to prepare biomolecules-based fluorescent fibers with both satisfactory biocompatibility and optimal mechanical properties. Here, we develop a fluorescent robust biofiber through using a tetraphenylethene-containing surfactant as the contact points between polysaccharide chains of alginate. This newly developed contact points not only strengthen the cross-linking network of polysaccharide chains, but also afford enough energy-dissipating slippage for polysaccharide chains. Consequently, the generated fluorescent fiber is endowed with highly improved mechanical performances from plastic strain stage. The experimental results indicate that the fluorescent fiber shows good mechanical properties of breaking strength of 1.10 GPa (12.09 cN/dtex), Young's modulus of 39.81 GPa and toughness of 137.26 MJ/m, which are comparable to those of dragline silk and outperforming spider silk proteins and other artificial materials. More importantly, its satisfactory biosafety and wound healing-promoting ability as a fluorescent suture are solidly proved by both in vitro and in vivo assays, which opens an opportunity for its biological and biomedical applications. This study provides a novel strategy for the development of robust fluorescent biomaterials.
新兴的研究注意力集中在开发用于各种生物医学应用的荧光生物材料上，包括荧光引导手术。然而，制备具有令人满意的生物相容性和最佳机械性能的基于生物分子的荧光纤维仍然具有挑战性。在这里，我们通过使用含有四苯乙烯的表面活性剂作为藻酸盐多糖链之间的接触点来开发一种荧光坚固的生物纤维。这种新开发的接触点不仅增强了多糖链的交联网络，而且为多糖链提供了足够的能量耗散滑移。因此，所产生的荧光纤维具有从塑性应变阶段开始的高度改善的机械性能。实验结果表明，荧光纤维表现出良好的机械性能，断裂强度为 1.10 GPa（12.09 cN/dtex），杨氏模量为 39.81 GPa，韧性为 137.26 MJ/m，可与拖丝和蜘蛛丝蛋白等其他人工材料相媲美。更重要的是，通过体外和体内实验，其作为荧光缝线的令人满意的生物安全性和促进伤口愈合能力得到了充分证实，这为其生物和生物医学应用开辟了机会。本研究为开发坚固的荧光生物材料提供了一种新策略。
