Department of Chemistry, School of Physical and Mathematical Sciences, University of Kerala, Kariavattom Campus, Thiruvananthapuram 695581, Kerala, India.
College of Food Science, Al-Qasim Green University, Babylon 51013, Iraq.
ACS Appl Bio Mater. 2024 Nov 18;7(11):7700-7709. doi: 10.1021/acsabm.4c01247. Epub 2024 Nov 1.
An economical, rapid, and ultrasensitive detection of biomolecules in clinical settings is very crucial, particularly for the early detection of Cardiac Troponin I (cTnI), which is the gold standard biomarker for Acute Myocardial Infarction (AMI). Electrochemiluminescence (ECL) has risen in prominence as an important technique for in vitro diagnosis and detection by virtue of its high sensitivity reaching a femtomolar level. This study introduces an economically feasible nanoplatform for ECL immunosensing, consisting of a gold nanoparticle (AuNP) with Ru(bpy) and tripropylamine (TPA) system, which is a potential ECL luminophore and coreactant system. AuNPs serve the role of an ECL signal enhancer as well as the carrier of antibody, which enables the creation of a label-free immunosensor for antigen-antibody interactions. The prepared immunosensor detected cTnI with a detection limit (LOD) of 0.03 ng/mL. This potential immunosensor provides appreciable results in the detection of cTnI from spiked real serum analysis, which shows its potential application in low-resource clinical settings.
在临床环境中，经济、快速和超灵敏地检测生物分子非常关键，特别是对于肌钙蛋白 I（cTnI）的早期检测，cTnI 是急性心肌梗死（AMI）的金标准生物标志物。由于其灵敏度达到飞摩尔级，电化学发光（ECL）作为一种重要的体外诊断和检测技术而备受关注。本研究提出了一种经济可行的用于 ECL 免疫传感的纳米平台，该平台由金纳米颗粒（AuNP）与钌（Ru）联吡啶（bpy）和三丙胺（TPA）组成，这是一种潜在的 ECL 发光体和共反应物系统。AuNPs 作为 ECL 信号增强剂和抗体的载体，可用于创建用于抗原-抗体相互作用的无标记免疫传感器。所制备的免疫传感器对 cTnI 的检测限（LOD）为 0.03ng/mL。该潜在的免疫传感器在检测加标实际血清分析中的 cTnI 方面取得了可观的结果，表明其在资源有限的临床环境中有潜在的应用。
