Hadroug Aldjia, Zengin Gokhan, Belhattab Rachid, Cakmak Yavuz S, Rebbas Khellaf
Department of Chemistry, Med Boudiaf University, M'sila, Algeria.
Laboratory of Applied Microbiology, Faculty of Life and Natural Sciences, Ferhat Abbas Setif1University, Setif, Algeria.
Nat Prod Res. 2024 Nov 2:1-8. doi: 10.1080/14786419.2024.2419498.
This research explored the impact of methanolic extracts from the fruits and leaves of L., a rare Algerian species, on several enzymes, focusing on tyrosinase, α-amylase, α-glucosidase, and cholinesterases, as well as their antioxidant potential assays, including phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, and MCA. HPLC-DAD analysis identified chlorogenic acid, rosmarinic acid, hesperidin, and quercetin as predominant phenolic components. Both extracts exhibited notable antioxidant properties, up to 250.08 mg TE/g for fruits and 237.08 mg TE/g for leaf extracts. Enzyme inhibitory assays demonstrated significant inhibition against AChE and BChE with maximum activities of 2.34 mg GALAE/g in leaves and 4.32 mg GALAE/g in fruits. Additionally, considerable tyrosinase inhibition was observed in leaves at 39.69 mg KAE/g. These findings highlight L. as a valuable source for investigating natural antioxidants and enzyme inhibitors, particularly for possible cosmetics and food additives applications.
本研究探讨了阿尔及利亚稀有物种L.的果实和叶子的甲醇提取物对几种酶的影响，重点关注酪氨酸酶、α-淀粉酶、α-葡萄糖苷酶和胆碱酯酶，以及它们的抗氧化潜力测定，包括磷钼酸法、DPPH法、ABTS法、CUPRAC法、FRAP法和MCA法。HPLC-DAD分析确定绿原酸、迷迭香酸、橙皮苷和槲皮素为主要酚类成分。两种提取物均表现出显著的抗氧化性能，果实提取物高达250.08mg TE/g，叶提取物高达237.08mg TE/g。酶抑制试验表明，对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）有显著抑制作用，叶中最大活性为2.34mg GALAE/g，果实中最大活性为4.32mg GALAE/g。此外，在叶中观察到对酪氨酸酶有相当大的抑制作用，为39.69mg KAE/g。这些发现突出了L.作为研究天然抗氧化剂和酶抑制剂的宝贵来源，特别是在可能的化妆品和食品添加剂应用方面。
