Filetti S, Vetri M, Damante G, Belfiore A
Endocrinology. 1986 Apr;118(4):1395-400. doi: 10.1210/endo-118-4-1395.
The nonmetabolizable glucose analogs, [3H]2-deoxy-D-glucose and [3H]O-methyl-D-glucose, were used to determine whether iodide influences glucose transport in porcine cells in primary culture. Incubation with iodide (3 h) decreased basal glucose transport with a half-maximum at NaI 3 X 10(-5) M and maximum at 10(-4) M. Iodide (10(-6) M to 10(-4) M) also abolished the stimulatory effect of TSH (1 mU/ml) on glucose transport. The iodide effect on [3H]2-deoxy-D-glucose transport had the following characteristics: 1) it was abolished 24 h after incubation in iodide-free medium; 2) it was prevented by methimazole (3 mM), and correlated with newly formed organic iodine, 3) and it affected the maximum velocity (Vmax) of glucose transport, reducing it from 25.1 to 14.4 and 12.0 nmol/(min mg protein) at 10(-5) M and 10(-4) M NaI, without affecting the Michaelis-Menten constant (Km) (6mM). Iodide-treated cells had a reduced specific binding of [3H]cytochalasin B (38% and 47% with respect to control cells at 10(-5) M and 10(-4) M NaI). These data suggest that iodide treatment reduces the functional carriers mediating glucose transport in the thyroid.
使用不可代谢的葡萄糖类似物[3H]2-脱氧-D-葡萄糖和[3H]O-甲基-D-葡萄糖来确定碘化物是否影响原代培养的猪细胞中的葡萄糖转运。用碘化物孵育（3小时）可降低基础葡萄糖转运，在碘化钠浓度为3×10(-5)M时达到半数最大效应，在10(-4)M时达到最大效应。碘化物（10(-6)M至10(-4)M）也消除了促甲状腺激素（1 mU/ml）对葡萄糖转运的刺激作用。碘化物对[3H]2-脱氧-D-葡萄糖转运的影响具有以下特点：1）在无碘化物培养基中孵育24小时后这种影响消失；2）甲巯咪唑（3 mM）可阻止这种影响，且与新形成的有机碘相关；3）它影响葡萄糖转运的最大速度（Vmax），在10(-5)M和10(-4)M碘化钠时，Vmax从25.1降至14.4和12.0 nmol/（分钟·毫克蛋白质），而不影响米氏常数（Km）（6 mM）。经碘化物处理的细胞对[3H]细胞松弛素B的特异性结合减少（在10(-5)M和10(-4)M碘化钠时，相对于对照细胞分别减少38%和47%）。这些数据表明，碘化物处理减少了甲状腺中介导葡萄糖转运的功能性载体。
