• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于免疫突触形成及嵌合抗原受体T细胞（CAR T细胞）对癌细胞激活的活细胞成像方案。

Protocol for live-cell imaging of immune synapse formation and activation of CAR T cells against cancer cells.

作者信息

Ibanez Jorge, Hebbar Nikhil, Thanekar Unmesha, Yi Zhongzhen, Houke Haley, Ward Meghan, Nevitt Chris, Tian Liqing, Mack Stephen C, Sheppard Heather, Chiang Jason, Velasquez M Paulina, Krenciute Giedre

机构信息

Department of Bone Marrow Transplantation and Cellular Therapy, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA.

Department of Developmental Neurobiology, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA.

出版信息

STAR Protoc. 2024 Dec 20;5(4):103422. doi: 10.1016/j.xpro.2024.103422. Epub 2024 Nov 1.

DOI:10.1016/j.xpro.2024.103422
PMID:39488837
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11566859/
Abstract

Immune synapse (IS) formation determines T cell antitumor activity. Here, we present a protocol for characterizing the IS formation between chimeric antigen receptor (CAR) T cells and tumor cells by measuring the IS size and calcium flux by live-cell imaging. We describe steps for CAR T cell manufacturing, sample preparation, image acquisition, and data analysis. For complete details on the use and execution of this protocol, please refer to Chockley et al., Ibanez et al., and Zoine et al..

摘要

免疫突触（IS）的形成决定了T细胞的抗肿瘤活性。在此，我们提供了一种通过活细胞成像测量IS大小和钙通量来表征嵌合抗原受体（CAR）T细胞与肿瘤细胞之间IS形成的方案。我们描述了CAR T细胞制造、样品制备、图像采集和数据分析的步骤。有关本方案使用和执行的完整详细信息，请参考乔克利等人、伊瓦涅斯等人和佐内等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7209/11566859/cdceab3883bc/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7209/11566859/21b9512fb860/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7209/11566859/040ab10ea91a/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7209/11566859/8f195ca471ae/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7209/11566859/77bc24fef5a4/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7209/11566859/6145c21c4609/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7209/11566859/cdceab3883bc/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7209/11566859/21b9512fb860/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7209/11566859/040ab10ea91a/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7209/11566859/8f195ca471ae/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7209/11566859/77bc24fef5a4/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7209/11566859/6145c21c4609/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7209/11566859/cdceab3883bc/gr5.jpg

相似文献

1
Protocol for live-cell imaging of immune synapse formation and activation of CAR T cells against cancer cells.用于免疫突触形成及嵌合抗原受体T细胞（CAR T细胞）对癌细胞激活的活细胞成像方案。
STAR Protoc. 2024 Dec 20;5(4):103422. doi: 10.1016/j.xpro.2024.103422. Epub 2024 Nov 1.
2
Granzyme B-activated IL18 potentiates αβ and γδ CAR T cell immunotherapy in a tumor-dependent manner.颗粒酶 B 激活的白介素 18 以肿瘤依赖性方式增强 αβ 和 γδ CAR T 细胞免疫疗法。
Mol Ther. 2024 Jul 3;32(7):2373-2392. doi: 10.1016/j.ymthe.2024.05.013. Epub 2024 May 14.
3
Biomimetic cell stimulation with a graphene oxide antigen-presenting platform for developing T cell-based therapies.用于开发基于T细胞疗法的具有氧化石墨烯抗原呈递平台的仿生细胞刺激
Nat Nanotechnol. 2024 Dec;19(12):1914-1922. doi: 10.1038/s41565-024-01781-4. Epub 2024 Sep 23.
4
Activation of Cell-Intrinsic Signaling in CAR-T Cells via a Chimeric IL7R Domain.通过嵌合 IL7R 结构域激活 CAR-T 细胞中的细胞内信号
Cancer Res Commun. 2024 Sep 1;4(9):2359-2373. doi: 10.1158/2767-9764.CRC-24-0286.
5
Chimeric antigen receptor (CAR) T-cell therapy for people with relapsed or refractory diffuse large B-cell lymphoma.嵌合抗原受体 (CAR) T 细胞疗法治疗复发或难治性弥漫性大 B 细胞淋巴瘤患者。
Cochrane Database Syst Rev. 2021 Sep 13;9(9):CD013365. doi: 10.1002/14651858.CD013365.pub2.
6
Bio-functional hydrogel coated membranes to decrease T-cell exhaustion in manufacturing of CAR T-cells.用于减少嵌合抗原受体T细胞制造过程中T细胞耗竭的生物功能水凝胶涂层膜。
Front Immunol. 2025 Jun 27;16:1513148. doi: 10.3389/fimmu.2025.1513148. eCollection 2025.
7
IL-18R supported CAR T cells targeting oncofetal tenascin C for the immunotherapy of pediatric sarcoma and brain tumors.IL-18R 支持的嵌合抗原受体 T 细胞针对癌胚 tenascin C 用于儿科肉瘤和脑肿瘤的免疫治疗。
J Immunother Cancer. 2024 Nov 20;12(11):e009743. doi: 10.1136/jitc-2024-009743.
8
Protocol to generate allorejection-resistant universal CAR-NKT cells and evaluate their efficacy, mechanism of action, safety, and immunogenicity.生成抗同种异体排斥通用型嵌合抗原受体自然杀伤T细胞并评估其疗效、作用机制、安全性和免疫原性的方案。
STAR Protoc. 2025 May 2;6(2):103810. doi: 10.1016/j.xpro.2025.103810.
9
CAR-T Cells Therapy in Glioblastoma: A Systematic Review on Molecular Targets and Treatment Strategies.嵌合抗原受体 T 细胞疗法治疗胶质母细胞瘤：分子靶点和治疗策略的系统评价。
Int J Mol Sci. 2024 Jun 29;25(13):7174. doi: 10.3390/ijms25137174.
10
Metabolic reprogramming via an engineered PGC-1α improves human chimeric antigen receptor T-cell therapy against solid tumors.通过工程化 PGC-1α 进行代谢重编程可改善针对实体瘤的人嵌合抗原受体 T 细胞疗法。
J Immunother Cancer. 2023 Mar;11(3). doi: 10.1136/jitc-2022-006522.

本文引用的文献

1
Peptide-scFv antigen recognition domains effectively confer CAR T cell multiantigen specificity.肽-scFv 抗原识别结构域能有效地赋予 CAR T 细胞多抗原特异性。
Cell Rep Med. 2024 Feb 20;5(2):101422. doi: 10.1016/j.xcrm.2024.101422. Epub 2024 Feb 12.
2
GRP78-CAR T cell effector function against solid and brain tumors is controlled by GRP78 expression on T cells.GRP78-CAR T 细胞对实体瘤和脑肿瘤的效应功能受 T 细胞上 GRP78 表达的控制。
Cell Rep Med. 2023 Nov 21;4(11):101297. doi: 10.1016/j.xcrm.2023.101297.
3
Nanoscale CAR Organization at the Immune Synapse Correlates with CAR-T Effector Functions.
纳米级 CAR 组织在免疫突触中与 CAR-T 效应功能相关。
Cells. 2023 Sep 12;12(18):2261. doi: 10.3390/cells12182261.
4
TrackMate 7: integrating state-of-the-art segmentation algorithms into tracking pipelines.TrackMate 7：将最先进的分割算法集成到跟踪管道中。
Nat Methods. 2022 Jul;19(7):829-832. doi: 10.1038/s41592-022-01507-1. Epub 2022 Jun 2.
5
CAR T cells redirected to cell surface GRP78 display robust anti-acute myeloid leukemia activity and do not target hematopoietic progenitor cells.嵌合抗原受体 T 细胞靶向细胞表面 GRP78 表现出强大的抗急性髓系白血病活性，并且不会靶向造血祖细胞。
Nat Commun. 2022 Jan 31;13(1):587. doi: 10.1038/s41467-022-28243-6.
6
Increasing LFA-1 Expression Enhances Immune Synapse Architecture and T Cell Receptor Signaling in Jurkat E6.1 Cells.增加淋巴细胞功能相关抗原-1（LFA-1）的表达可增强Jurkat E6.1细胞中的免疫突触结构和T细胞受体信号传导。
Front Cell Dev Biol. 2021 Jul 23;9:673446. doi: 10.3389/fcell.2021.673446. eCollection 2021.
7
Patient-derived models recapitulate heterogeneity of molecular signatures and drug response in pediatric high-grade glioma.患者来源模型再现了小儿高级别胶质瘤分子特征和药物反应的异质性。
Nat Commun. 2021 Jul 2;12(1):4089. doi: 10.1038/s41467-021-24168-8.
8
H3.3 K27M depletion increases differentiation and extends latency of diffuse intrinsic pontine glioma growth in vivo.H3.3 K27M 耗竭增加了弥漫性内在脑桥神经胶质瘤在体内的分化并延长了潜伏期。
Acta Neuropathol. 2019 Apr;137(4):637-655. doi: 10.1007/s00401-019-01975-4. Epub 2019 Feb 15.
9
Phototoxicity in live fluorescence microscopy, and how to avoid it.活细胞荧光显微镜检查中的光毒性及其规避方法。
Bioessays. 2017 Aug;39(8). doi: 10.1002/bies.201700003.
10
Immunological Consequences of Epithelial-Mesenchymal Transition in Tumor Progression.肿瘤进展过程中上皮-间质转化的免疫后果
J Immunol. 2016 Aug 1;197(3):691-8. doi: 10.4049/jimmunol.1600458.