Cheng Chunfang, Han Fangqin, Zhou Hengyu, Wang Huixin, Zhao Jingyu, Zhao Guanhui, Zhang Yong, Zhang Nuo, Wang Yaoguang, Luan Mingming, Wei Qin
Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China.
School of Chemistry and Chemical Engineering, Qilu Normal University, Jinan, 250200, China.
Talanta. 2025 Feb 1;283:127147. doi: 10.1016/j.talanta.2024.127147. Epub 2024 Nov 2.
Cancer is currently one of the major causes of human mortality and has received widespread attention. In this paper, Au@CuS composite nanomaterial as a sandwich immunosensor tag for carcinoembryonic antigen (CEA) detection strategy was studied. Herein, Au@CuS composite nanomaterials were obtained by Au nanoparticles modified with CuS, which were combined with secondary antibody (Ab) to construct an immunosensor that interacted with HO to produce a current response. The anti-CEA primary antibody (Ab) was fixed on the glassy carbon electrode (GCE) modified by Au@MXene. The completed electrochemical immunosensor was constructed with rapid detection and high sensitivity for CEA. Under optimal conditions, the linearity ranged from 0.01 pg/mL to 0.5 ng/mL, and the detection limit was 3.8 fg/mL. This tactic possesses good reproducibility, constancy and selectivity. At the same time, this strategy has latent practical value for the test of other tumor markers.
癌症是目前人类死亡的主要原因之一，已受到广泛关注。本文研究了以Au@CuS复合纳米材料作为夹心免疫传感器标签用于癌胚抗原（CEA）检测的策略。在此，通过用CuS修饰Au纳米颗粒获得Au@CuS复合纳米材料，其与二抗（Ab）结合构建免疫传感器，该免疫传感器与HO相互作用产生电流响应。抗CEA一抗（Ab）固定在经Au@MXene修饰的玻碳电极（GCE）上。所构建的电化学免疫传感器对CEA具有快速检测和高灵敏度。在最佳条件下，线性范围为0.01 pg/mL至0.5 ng/mL，检测限为3.8 fg/mL。该策略具有良好的重现性、稳定性和选择性。同时，该策略对其他肿瘤标志物的检测具有潜在的实用价值。
