Gupta Tanya, Lal Kanhaiya, Singh Randhir
Department of Pharmacology, Central University of Punjab, Ghudda, Bathinda-151401, India.
J Ethnopharmacol. 2025 Feb 10;338(Pt 1):119021. doi: 10.1016/j.jep.2024.119021. Epub 2024 Nov 1.
Astilbe rivularis Buch.-Ham. ex D. Don is a rare medicinal plant, traditionally employed for treating several disorders. The juice, decoction or powder of the roots, rhizomes, leaves and even the entire plant, are used for managing peptic ulcer, diarrhoea, jaundice, sprains and muscular swellings, bone fracture and dislocation of joints, postpartum bleeding and other menstrual disorders. These conventional medicinal uses make Astilbe rivularis a promising candidate for further research.
This study was designed to explore the neuroprotective potential of hydroethanolic extract of Astilbe rivularis (ARHE) in diabetic neuropathy (DN) in rats.
GC-MS analysis was used to identify the phytoconstituents present in the plant extract. DN was induced by administration of STZ (55 mg/kg, i.p.), 15 min after NAD (230 mg/kg, i.p.) injection. The rats with fasting blood glucose (FBG) level >250 mg/dl were included in the study. DN was assessed by estimating the level of FBG, lipid profile, and invitro and invivo oxidative stress parameters. Additionally, behavioural parameters like, mechanical hyperalgesia, hot and cold allodynia were estimated to assess diabetic neuropathy. Furthermore, the level of antioxidant enzymes like SOD, GSH, and TBARS in sciatic nerve and inflammatory markers like, TGF-β and IL-6 were measured.
Altogether, 30 phytoconstituents were identified including heptafluorobutyric acid, hexadecanoic acid, and beta-sitosterol depicting antioxidant, antidiabetic, and anticancer properties, respectively. Administration of different doses (100, 200, and 400 mg/kg) of ARHE to diabetic rats attenuated elevated blood glucose level and restored lipid profile, body weight, food and water intake, and antioxidant level. Moreover, elevated level of inflammatory markers like, TGF-β and IL-6 was also found to be attenuated in sciatic nerve. Furthermore, ARHE attenuated the pain response assessed by mechanical hyperalgesia and hot and cold allodynia in diabetic neuropathy rats. ARHE also showed inhibitory activity on ALR enzyme and erythrocyte sorbitol accumulation, and ameliorated oxidative stress. Histopathological study indicated improvement in the architecture of sciatic nerve tissue in diabetic neuropathy rats with the treatment of ARHE.
Conclusively, hydroethanolic extract of Astilbe rivularis exhibited neuroprotective potential and ameliorated diabetic neuropathy in rats.
溪畔落新妇是一种珍稀药用植物，传统上用于治疗多种疾病。其根、根茎、叶甚至全株的汁液、煎剂或粉末，可用于治疗消化性溃疡、腹泻、黄疸、扭伤和肌肉肿胀、骨折和关节脱位、产后出血及其他月经失调。这些传统药用价值使溪畔落新妇成为进一步研究的理想候选植物。
本研究旨在探讨溪畔落新妇水乙醇提取物（ARHE）对大鼠糖尿病性神经病变（DN）的神经保护潜力。
采用气相色谱 - 质谱联用（GC - MS）分析鉴定植物提取物中的植物成分。在腹腔注射烟酰胺（NAD，230 mg/kg）15分钟后，腹腔注射链脲佐菌素（STZ，55 mg/kg）诱导糖尿病性神经病变。空腹血糖（FBG）水平>250 mg/dl的大鼠纳入研究。通过评估FBG水平、血脂谱以及体内外氧化应激参数来评估糖尿病性神经病变。此外，还评估了机械性痛觉过敏、冷热痛觉异常等行为学参数以评估糖尿病性神经病变。此外，还测定了坐骨神经中抗氧化酶如超氧化物歧化酶（SOD）、谷胱甘肽（GSH）和丙二醛（TBARS）的水平以及炎症标志物如转化生长因子 -β（TGF -β）和白细胞介素 -6（IL -6）的水平。
共鉴定出30种植物成分，包括七氟丁酸、十六烷酸和β - 谷甾醇，分别具有抗氧化、抗糖尿病和抗癌特性。给糖尿病大鼠给予不同剂量（100、200和400 mg/kg）的ARHE可降低血糖水平，恢复血脂谱、体重、食物和水摄入量以及抗氧化水平。此外，还发现坐骨神经中炎症标志物如TGF -β和IL -6的水平也有所降低。此外，ARHE减轻了糖尿病性神经病变大鼠通过机械性痛觉过敏以及冷热痛觉异常评估的疼痛反应。ARHE还对醛糖还原酶（ALR）酶和红细胞山梨醇积累具有抑制活性，并改善了氧化应激。组织病理学研究表明，用ARHE治疗的糖尿病性神经病变大鼠坐骨神经组织的结构有所改善。
总之，溪畔落新妇水乙醇提取物在大鼠中表现出神经保护潜力并改善了糖尿病性神经病变。
