Park Jiwoo, Kim Donghee, Kwon Myeongjee, Kwon Hyun, Yong Jungyeon, Yoon Haerin, Hwang Jiyi, Jung Ji Seung, Park Kyung-Mee
Laboratory of Veterinary Surgery and Ophthalmology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Vet Ophthalmol. 2024 Nov 3. doi: 10.1111/vop.13296.
To analyze bacterial isolates associated with canine bacterial keratitis and their antibiotic susceptibility patterns in Korea, focusing on multidrug resistance (MDR) and identifying effective antibiotic combinations for clinical treatment.
A total of 146 dogs diagnosed with suspected bacterial keratitis between October 2022 and October 2023 in Korea, with 157 eye samples collected for analysis.
Eye samples were cultured to isolate bacteria, and antibiotic susceptibility testing was performed using the minimum inhibitory concentration (MIC) method. Bacterial identification was conducted using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF). The study assessed the efficacy of individual antibiotics and combination therapies.
Bacteria were isolated in 55.4% of the samples. The most common genera were Staphylococcus species (48.5%, 48/99), Streptococcus species (13.1%, 13/99), Pseudomonas species (9.1%, 9/99), and Escherichia coli (9.1%, 9/99). Amikacin (84.8%) showed the highest antibiotic susceptibility, while doxycycline exhibited the lowest (17.2%). The most effective antibiotic combinations were amikacin-moxifloxacin (93%). MDR isolates accounted for 52.5% (52/99) of the total bacterial samples.
Staphylococcus species were the most common isolates, with 52.5% showing MDR, underscoring the need to curb antibiotic misuse. While antibiotics like amikacin demonstrated high susceptibility rates, their use should be reserved for resistant infections to prevent further resistance development. Rather than focusing solely on finding effective combinations of antibiotics, it is crucial to consider alternative treatment strategies that offer more sustainable solutions. Rather than relying on antibiotic combinations, attention should shift to sustainable alternatives to treat bacterial keratitis and reduce antibiotic dependence in clinical practice.
分析韩国犬细菌性角膜炎相关的细菌分离株及其抗生素敏感性模式，重点关注多重耐药性（MDR），并确定临床治疗的有效抗生素组合。
2022年10月至2023年10月期间在韩国共146只被诊断为疑似细菌性角膜炎的犬，收集了157份眼样本进行分析。
对眼样本进行培养以分离细菌，并使用最低抑菌浓度（MIC）法进行抗生素敏感性测试。使用基质辅助激光解吸/电离飞行时间（MALDI-TOF）进行细菌鉴定。该研究评估了单一抗生素和联合疗法的疗效。
55.4%的样本中分离出细菌。最常见的菌属是葡萄球菌属（48.5%，48/99）、链球菌属（13.1%，13/99）、假单胞菌属（9.1%，9/99）和大肠杆菌（9.1%，9/99）。阿米卡星（84.8%）显示出最高的抗生素敏感性，而强力霉素最低（17.2%）。最有效的抗生素组合是阿米卡星-莫西沙星（93%）。多重耐药分离株占细菌样本总数的52.5%（52/99）。
葡萄球菌属是最常见的分离株，52.5%表现出多重耐药性，这凸显了遏制抗生素滥用的必要性。虽然阿米卡星等抗生素显示出高敏感性，但应仅用于耐药感染，以防止进一步产生耐药性。与其仅仅专注于寻找有效的抗生素组合，考虑提供更可持续解决方案的替代治疗策略至关重要。不应仅仅依赖抗生素组合，而应将注意力转向治疗细菌性角膜炎的可持续替代方法，并在临床实践中减少对抗生素的依赖。
