Tianjin Medical University, Tianjin, China.
School of Physics, Nankai University, Tianjin, China.
Biomater Adv. 2025 Jan;166:214080. doi: 10.1016/j.bioadv.2024.214080. Epub 2024 Oct 20.
Bone fractures are frequently encountered in clinical practice and pose significant challenges, due to the high incidence of delayed healing or non-union. Reduced graphene oxide (rGO) stands out for its unique sensitivity to a wide range of light wavelengths, especially in the near-infrared (NIR) spectrum. In this study, we developed an approach to promote fracture healing using an rGO-HAMC hydrogel activated by NIR stimulation. In vitro experiments demonstrated that NIR-stimulated rGO hydrogel significantly enhanced osteogenesis in bone marrow stromal cells (BMSCs). In an in vivo mouse fracture model, the rGO hydrogel was applied to the fracture site, and NIR light was delivered transdermally, showing promising results in accelerating bone regeneration. These results demonstrate that NIR-stimulated rGO hydrogel significantly accelerates fracture healing, as evidenced by increased bone volume and trabecular thickness. Additionally, transcriptomic analysis revealed that pathways related to B-cell activation are implicated in the enhanced healing effects mediated by NIR-stimulated rGO hydrogel. These findings highlight the potential of the NIR-stimulated rGO hydrogel as an innovative strategy for bone regeneration, offering promising new avenues for the treatment and management of bone fractures.
骨骨折在临床实践中经常遇到，由于愈合延迟或不愈合的发生率较高，因此带来了重大挑战。还原氧化石墨烯（rGO）因其对广泛的光波长具有独特的敏感性而脱颖而出，尤其是在近红外（NIR）光谱中。在这项研究中，我们开发了一种使用 NIR 刺激激活的 rGO-HAMC 水凝胶促进骨折愈合的方法。体外实验表明，NIR 刺激的 rGO 水凝胶可显著增强骨髓基质细胞（BMSCs）中的成骨作用。在体内小鼠骨折模型中，将 rGO 水凝胶应用于骨折部位，并经皮传递 NIR 光，结果表明其在加速骨再生方面具有很大的潜力。这些结果表明，NIR 刺激的 rGO 水凝胶可显著加速骨折愈合，这可通过增加骨体积和小梁厚度来证明。此外，转录组分析表明，与 B 细胞激活相关的途径与 NIR 刺激的 rGO 水凝胶介导的增强愈合作用有关。这些发现突出了 NIR 刺激的 rGO 水凝胶作为骨再生的创新策略的潜力，为骨骨折的治疗和管理提供了有希望的新途径。
