全转录组测序分析揭示 circZFYVE9/miR-378a-3p/IMMT 轴对脂肪细胞中线粒体功能的影响。

Whole transcriptome sequencing analysis reveals the effect of circZFYVE9/miR-378a-3p/IMMT axis on mitochondrial function in adipocytes.

机构信息

State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.

出版信息

Int J Biol Macromol. 2024 Nov;281(Pt 2):136916. doi: 10.1016/j.ijbiomac.2024.136916. Epub 2024 Oct 26.

DOI:10.1016/j.ijbiomac.2024.136916

PMID:39490878

Abstract

Recent research highlights the complex regulation of lipid accumulation and mitochondrial function in adipocytes via non-coding RNAs like microRNAs and circular non-coding RNAs. Circular non-coding RNAs act as endogenous regulators, impacting lipid metabolism and mitochondrial function by interacting with miRNAs. Sequencing white and brown adipose tissues in mice revealed significant variations in 1936 mRNAs, 127 miRNAs, and 171 circRNAs. Analyses showed these RNAs' involvement in vital processes like mitochondrial biogenesis, oxidative phosphorylation, and the citric acid cycle, crucial for lipid metabolism. Focus on top differentially regulated miRNAs led to the construction of a regulatory network involving circRNAs, miRNAs, and mRNAs, illuminating the role of endogenous RNAs in lipid metabolism and mitochondrial function. The circZFYVE9/miR-378a-3p/IMMT axis was identified as influential in adipogenic differentiation of 3T3-L1 preadipocytes by regulating mitochondrial function. This study expands the understanding of non-coding RNAs in adipose tissue, particularly their connection to mitochondrial function and metabolism.

摘要

最近的研究强调了非编码 RNA （如 microRNAs 和环状非编码 RNA）在脂肪细胞中脂质积累和线粒体功能的复杂调节作用。环状非编码 RNA 作为内源性调节剂，通过与 microRNAs 相互作用，影响脂质代谢和线粒体功能。对小鼠白色和棕色脂肪组织进行测序，揭示了 1936 个 mRNAs、127 个 miRNAs 和 171 个 circRNAs 的显著变化。分析表明，这些 RNA 参与了线粒体生物发生、氧化磷酸化和柠檬酸循环等重要过程，这些过程对脂质代谢至关重要。对差异表达 miRNA 的重点分析导致构建了一个涉及 circRNAs、miRNAs 和 mRNAs 的调控网络，阐明了内源性 RNA 在脂质代谢和线粒体功能中的作用。circZFYVE9/miR-378a-3p/IMMT 轴被确定为影响 3T3-L1 前体脂肪细胞成脂分化的关键因素，通过调节线粒体功能。这项研究扩展了对脂肪组织中非编码 RNA 的理解，特别是它们与线粒体功能和代谢的联系。