一种基于流式细胞术的检测方法，用于评估小鼠黏膜相关恒定T细胞（MAIT细胞）和自然杀伤细胞联合作用对肿瘤细胞的细胞溶解活性。

Sugimoto Chie, Fujita Hiroyoshi, Wakao Hiroshi

Host Defense Division, Research Center for Advanced Medical Science, Dokkyo Medical University, Mibu, Tochigi 321-0293, Japan.

STAR Protoc. 2023 Oct 27;4(4):102620. doi: 10.1016/j.xpro.2023.102620.

Mucosal-associated invariant T (MAIT) cells are innate-like T cells responsible for mucosal immunity in the respiratory and intestinal tracts. Here we present a flow-cytometry-based assay to measure the cytolytic activity of murine MAIT cells and natural killer (NK) cells. We describe steps for differentiating MAIT-like cells from the induced pluripotent stem cells prepared from MAIT cells (reMAIT cells), NK cell isolation, co-culture with target tumor cells, and staining to distinguish dead cells from live cells. For complete details on the use and execution of this protocol, please refer to Sugimoto et al. (2022)..

黏膜相关恒定T（MAIT）细胞是一类固有样T细胞，负责呼吸道和肠道的黏膜免疫。在此，我们介绍一种基于流式细胞术的检测方法，用于测量小鼠MAIT细胞和自然杀伤（NK）细胞的细胞溶解活性。我们描述了从MAIT细胞制备的诱导多能干细胞（reMAIT细胞）中分化出MAIT样细胞、NK细胞分离、与靶肿瘤细胞共培养以及染色以区分死细胞和活细胞的步骤。有关本方案使用和执行的完整详细信息，请参阅杉本等人（2022年）的文献。