Institute of Entomology, The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Guizhou Univerisity, Gui Yang, China.
Research Center of Buckwheat Industry Technology, School of Life Science, Guizhou Normal University, Gui Yang, China.
PeerJ. 2024 Oct 29;12:e18345. doi: 10.7717/peerj.18345. eCollection 2024.
is one of the most serious pests of buckwheat, with its larvae mainly damaging leaves during the seedling and flowering stages. Entomogenous fungi play a crucial role as biological regulators of arthropod populations in nature. In this paper, a newly isolated strain 19GZAl-1, was identified as through the observetions of macroscopic and microscopic morphological features, and the results of rDNA- ITS sequence alignment and phylogenetic analysis. By comparing the efficacy of ten entomopathogenic fungal strains, including six strains of and four other species, against larvae, the new strain 19GZAl-1 exhibited the highest mortality rate. Mortality rates of larvae treated by spraying with the strain 19GZAl-1 increased as larval stage decreased, with first instar larvae showing the highest mortality rate of 85%. The appressorium from conidia invaded the larval body through areas with thin cuticle, such as larva pod base and internode folds, after which the hyphae grew rapidly, filling the haemocoel, and finally protruding from the integument upon melanization and decomposition of the intestinal wall cells. The results suggest that the strain 19GZAL-1 of has potential against larvae, which is significant for further study on the infection mechanism of on insects.
是荞麦上最严重的害虫之一，其幼虫主要在幼苗和开花期损害叶片。昆虫病原真菌在自然界中作为节肢动物种群的生物调节剂起着至关重要的作用。在本文中，通过观察宏观和微观形态特征，以及 rDNA-ITS 序列比对和系统发育分析的结果，鉴定新分离的菌株 19GZAl-1 为 。通过比较十种昆虫病原真菌菌株（包括六种 菌株和四种其他 种）对 幼虫的防治效果，新菌株 19GZAl-1 表现出最高的死亡率。用菌株 19GZAl-1 喷雾处理的幼虫死亡率随着幼虫阶段的降低而增加，第一龄幼虫的死亡率最高，达到 85%。分生孢子的附着胞通过幼虫体壁较薄的区域（如幼虫荚基部和节间褶皱）侵入幼虫体，然后菌丝迅速生长，充满血腔，最后在肠壁细胞黑化和分解时从体壁突出。结果表明，菌株 19GZAL-1 对 幼虫具有潜在的防治作用，这对进一步研究 对昆虫的感染机制具有重要意义。
