Colección de Hongos Entomopatógenos, Centro Nacional de Referencia de Control Biológico, Tecomán, Colima, México.
Instituto de Farmacobiología, Universidad de la Cañada, Teotitlán de Flores Magón, Oaxaca, México.
J Basic Microbiol. 2021 Feb;61(2):147-156. doi: 10.1002/jobm.202000627. Epub 2021 Jan 14.
Media formulated with insect cuticle (0.5% and 1%; Sphequit Sph®), with a reduction in nutrients (¼ Sabouraud dextrose agar + yeast [SDAY]) and commercial media (potato dextrose agar, Sabouraud dextrose agar) were evaluated for the cultivation of Beauveria bassiana, Cordyceps javanica (Isaria javanica [Bally] Samson & Hywel-Jones), and Metarhizium robertsii. By using principal component analysis, it was determined that the ¼ SDAY and Sph formulations have greater advantages than commercial media for the development of fungi. The ¼ SDAY and Sph (0.5% and 1%) improved hydrophobicity, radial growth rate, germination, conidia yield, and virulence in B. bassiana; in M. robertsii, they favored conidia yield, germination, and virulence, and in C. javanica, the ¼ SDAY and Sph 0.5% media enhanced conidia yield, germination, radial growth rate, and virulence. We suggest that these formulations are an alternative to commercial culture media as they are cheaper and appropriate to improve the growth characteristics and virulence of the three strains evaluated. Some applications of culture media are suggested, and the importance of multivariate analysis as an exploratory tool to carry out the choice of culture media in a suitable way for the development of mycoinsecticides is also discussed.
用昆虫表皮（0.5%和 1%；Sphequit Sph®）制成的培养基、减少营养物质（¼ 沙氏葡萄糖琼脂+酵母[SDAY]）和商业培养基（土豆葡萄糖琼脂、沙氏葡萄糖琼脂）被评估用于培养球孢白僵菌、中华被毛孢（拟青霉[Bally] Samson & Hywel-Jones）和金龟子绿僵菌。通过主成分分析，确定¼ SDAY 和 Sph 配方比商业培养基更有利于真菌的生长。¼ SDAY 和 Sph（0.5%和 1%）提高了球孢白僵菌的疏水性、径向生长率、萌发、分生孢子产量和毒力；在绿僵菌中，它们有利于分生孢子产量、萌发和毒力；在中华被毛孢中，¼ SDAY 和 Sph 0.5%培养基增强了分生孢子产量、萌发、径向生长率和毒力。我们建议这些配方是商业培养基的替代品，因为它们更便宜，适合提高三种评估菌株的生长特性和毒力。还提出了一些培养基的应用建议，并讨论了多元分析作为一种探索性工具的重要性，以便以合适的方式选择适合开发生防制剂的培养基。
