Department of Ophthalmology, Professor K. Gibinski University Clinical Center, Medical University of Silesia, Katowice, Poland.
Department of Biochemistry, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Zabrze, Poland.
Acta Biochim Pol. 2024 Oct 18;71:13581. doi: 10.3389/abp.2024.13581. eCollection 2024.
Oxidative stress is an important factor for vitreomacular interface disease development in a theoretical model.
The aim of the study was to evaluate the correlation between oxidative stress in the human epiretinal membrane (ERM) and retinal morphological changes.
The study included patients scheduled for vitrectomy with epiretinal membrane removal. LogMAR best corrected visual acuity was assessed and optical coherence tomography was performed. Patients were divided into three groups: Type 1 - epiretinal membrane with premacular fibrosis; type 2 - epiretinal membrane with co-existing layer hole; and type 3 - ERM with co-existing full-thickness macular hole. During vitrectomy, epiretinal membranes were collected. Total oxidant status was determined by an automated colorimetric method in homogenates of epiretinal membrane.
The Mann-Whitney U test, Kruskal-Wallis test and Spearman linear correlation analysis were used. Statistical significance was set with a level of α = 0.05.
Twenty-one Caucasian women (60%) and 14 men (40%) were included in the study. The average age of participants was 74.7 years (95% CI: 71.13-75.45). The mean best corrected visual acuity LogMAR value in the group was 0.8 (95% CI: 0.9-0.7). The mean ratio of total oxidant status to protein level in the collected samples was 0.161 (95% CI: 0.08-0.23) µmol/mg of protein. No correlation was found between total oxidant status and the degree of morphological retinal changes.
The study found no significant correlation between the level of oxidative stress in epiretinal membrane and retinal morphological changes.
在理论模型中，氧化应激是玻璃体黄斑界面疾病发展的一个重要因素。
本研究旨在评估人视网膜内表面膜（ERM）中的氧化应激与视网膜形态变化之间的相关性。
该研究纳入了计划接受玻璃体切除术和视网膜内表面膜切除的患者。评估最佳矫正视力对数视力（LogMAR），并进行光学相干断层扫描（OCT）。患者分为三组：1 型 - 黄斑前膜伴纤维增生；2 型 - 黄斑前膜伴层间孔并存；3 型 - ERM 伴全层黄斑孔并存。在玻璃体切除术中，收集视网膜内表面膜。通过自动比色法在视网膜内表面膜匀浆中测定总氧化剂状态。
采用 Mann-Whitney U 检验、Kruskal-Wallis 检验和 Spearman 线性相关分析。统计学意义设定为 α = 0.05。
本研究纳入 21 名白种女性（60%）和 14 名男性（40%）。参与者的平均年龄为 74.7 岁（95%可信区间：71.13-75.45）。该组平均最佳矫正视力 LogMAR 值为 0.8（95%可信区间：0.9-0.7）。收集样本中总氧化剂状态与蛋白质水平的比值平均为 0.161（95%可信区间：0.08-0.23）µmol/mg 蛋白质。未发现总氧化剂状态与视网膜形态变化程度之间存在相关性。
本研究未发现视网膜内表面膜中氧化应激水平与视网膜形态变化之间存在显著相关性。
