Department of Analytical Chemistry, Faculty of Chemistry and Pharmacy, Opole University, Oleska 48, 45-052, Opole, Poland.
Faculty of Chemistry, Adam Mickiewicz University in Poznan, Uniwersytetu Poznańskiego 8, 61-614, Poznań, Poland.
Sci Rep. 2024 Nov 4;14(1):26614. doi: 10.1038/s41598-024-74180-3.
Biostimulants that improve agriculture's productivity and environmental responsibility are widely favored and used. Algae has emerged as a viable option for sustainable agriculture. In the present study, the effects of the Cladophora glomerata (L.) Kütz. aqueous extract in three concentrations (2.5, 5, and 10 mg extract per mL of tap water) was tested on the growth and productivity of garden cress (GCR) Lepidium sativum L., plants over 12 days, and the results were compared with those of the control group (irrigated only with tap water). The effect of the algae extract was studied by assessing the changes in phenolic compounds using HPLC-DAD and determining the chlorophyll content as well as the total antioxidant capacity of the GCR using DPPH and ABTS tests. Total phenolics, as well as total flavonoids, were measured. The potential role of algae extract in promoting cress plant growth was attained when compared to the control, mainly at a concentration of 2.5 mg/mL, which exhibited the highest yield growth after 12 days and presented the highest antioxidant capacity at 13.53 ± 1.16 mg Trolox equivalent (TE)/g dry weight (DW), compared to 10.44 ± 0.33 mgTE/g DW for the control. The total phenolic content significantly increased (p < 0.05) from 14.34 ± 0.84 mg gallic acid equivalent (GAE)/ g dry weight (DW) to 23.14 ± 0.55 mg GAE/g DW. Eleven phenolic compounds were identified in different tested samples of GCR, whether treated or not treated with algae extracts. Chrysin was only identified in the treated plants. Therefore, the use of algae (C. glomerata) presents promising potential as a biostimulant in agriculture, contributing to increased plant growth and improved resistance to environmental stress.
生物刺激剂能够提高农业的生产力和环境责任感，因此受到广泛的青睐和应用。藻类已成为可持续农业的可行选择。在本研究中，我们测试了三种浓度（每毫升自来水 2.5、5 和 10 毫克提取物）的团藻（Cladophora glomerata（L.）Kütz.）水提物对芫荽（GCR）荠（Lepidium sativum L.）植物生长和生产力的影响，为期 12 天，并将结果与仅用自来水灌溉的对照组进行了比较。通过使用 HPLC-DAD 评估酚类化合物的变化以及使用 DPPH 和 ABTS 测试测定叶绿素含量和 GCR 的总抗氧化能力来研究藻类提取物的作用。测量了总酚和总类黄酮的含量。与对照组相比，当藻类提取物浓度为 2.5mg/mL 时，对促进荠植物生长的效果最为显著，在 12 天后表现出最高的产量生长，并表现出最高的抗氧化能力，为 13.53±1.16mg Trolox 当量（TE）/g 干重（DW），而对照组为 10.44±0.33mgTE/g DW。总酚含量显著增加（p<0.05），从 14.34±0.84mg 没食子酸当量（GAE）/g 干重（DW）增加到 23.14±0.55mg GAE/g DW。在未经或经藻类提取物处理的 GCR 不同测试样本中鉴定出 11 种酚类化合物，无论是处理过还是未处理过的。白杨素仅在处理过的植物中被鉴定出来。因此，藻类（C. glomerata）的使用具有作为农业生物刺激剂的巨大潜力，可以促进植物生长并提高对环境胁迫的抵抗力。
