Department of Endocrinology, School of Medicine, Chongqing University Three Gorges Hospital, Chongqing University, Chongqing, 404100, China.
The Center of Clinical Research of Endocrinology and Metabolic Diseases in Chongqing, Chongqing University Three Gorges Hospital, Chongqing, 404100, China.
Sci Rep. 2024 Nov 4;14(1):26703. doi: 10.1038/s41598-024-77453-z.
The present study investigated the Zinc finger BED-type containing 3 (ZBED3) mRNA expression levels, diagnostic and prognostic values, co-expressed and differentially expressed genes, immune infiltration, and functional enrichment analysis in patients with kidney renal clear cell carcinoma (KIRC) using various public databases such as The Cancer Genome Atlas, Genotype-Tissue Expression Project, LinkedOmics, Gene Set Enrichment Analysis databases, and the Xiantao academic tool. Through extensive data mining, our study revealed differential expression of ZBED3 in various tumor and paired normal tissues. Specifically, ZBED3 expression was found to be significantly decreased in KIRC, particularly in advanced pathologic stages and histologic grades, when compared to corresponding control tissues. Our analysis revealed that the overexpression of ZBED3 is associated with a favorable overall survival outcome in KIRC patients. Moreover, functional enrichment analysis identified key pathways related to inflammation response and DNA methylation of ZBED3 in KIRC. Additionally, our findings suggest that the upregulation of ZBED3 leads to the inhibition of cell proliferation by modulating cell-cycle progression in KIRC cell lines. Taken together, these results suggest that ZBED3 may serve as a promising biomarker and prognostic indicator for individuals diagnosed with KIRC.
本研究利用癌症基因组图谱、组织表达项目、LinkedOmics、基因集富集分析数据库和仙桃学术工具等多种公共数据库，研究了锌指 BED 型包含 3（ZBED3）mRNA 表达水平、诊断和预后价值、共表达和差异表达基因、免疫浸润和功能富集分析在肾透明细胞癌（KIRC）患者中的作用。通过广泛的数据挖掘，我们的研究揭示了 ZBED3 在各种肿瘤和配对正常组织中的差异表达。具体来说，与相应的对照组织相比，ZBED3 在 KIRC 中表达明显降低，特别是在晚期病理阶段和组织学分级中。我们的分析表明，ZBED3 的过表达与 KIRC 患者的总生存结局良好相关。此外，功能富集分析确定了与 KIRC 中 ZBED3 的炎症反应和 DNA 甲基化相关的关键途径。此外，我们的研究结果表明，ZBED3 的上调通过调节 KIRC 细胞系的细胞周期进展来抑制细胞增殖。综上所述，这些结果表明 ZBED3 可能作为 KIRC 患者的一种有前途的生物标志物和预后指标。
