Animal Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.
BMC Genomics. 2024 Nov 4;25(1):1029. doi: 10.1186/s12864-024-10836-8.
Chickens play a crucial role as the primary global source of eggs and poultry, and the quality of rooster semen significantly impacts poultry reproductive efficiency. Therefore, it is imperative to comprehend the regulatory mechanisms underlying sperm development.
In this study, we established transcriptome profiles of lncRNAs, miRNAs, and mRNAs in 3 testis tissues and 3 epididymis tissues from "Jing Hong No.1" roosters at 24, 35, and 64 weeks of age. Using the data, we conducted whole transcriptome analysis and constructed a ceRNA network. We detected 10 differentially expressed mRNAs (DEmRNAs), 33 differentially expressed lncRNAs (DElncRNAs), and 10 differentially expressed miRNAs (DEmiRNAs) in the testis, as well as 149 DEmRNAs, 12 DElncRNAs, and 10 DEmiRNAs in the epididymis. These genes were found to be involved in cell differentiation and development, as well as various signaling pathways such as GnRH, MAPK, TGF-β, mTOR, VEGF, and calcium ion pathways. Subsequently, we constructed two competing endogenous RNA (ceRNA) networks comprising DEmRNAs, DElncRNAs, and DEmiRNAs. Furthermore, we identified four crucial lncRNA-mRNA-miRNA interactions that govern specific biological processes in the chicken reproductive system: MSTRG.2423.1-gga-miR-1563-PPP3CA and MSTRG.10064.2-gga-miR-32-5p-GPR12 regulating sperm motility in the testis; MSTRG.152556.1-gga-miR-9-3p-GREM1/THYN1 governing immunomodulation in the epididymis; and MSTRG.124708.1-gga-miR-375-NDUFB9/YBX1 controlling epididymal sperm maturation and motility.
Whole transcriptome sequencing of chicken testis and epididymis screened several key genes and ceRNA regulatory networks, which may be involved in the regulation of epididymal immunity, spermatogenesis and sperm viability through the pathways of MAPK, TGF-β, mTOR, and calcium ion. These findings contribute to our comprehensive understanding of the intricate molecular processes underlying rooster spermatogenesis, maturation and motility.
鸡是全球鸡蛋和家禽的主要来源，公鸡精液的质量对家禽繁殖效率有重要影响。因此，了解精子发育的调控机制至关重要。
本研究以“京红 1 号”公鸡 24、35 和 64 周龄的 3 个睾丸组织和 3 个附睾组织为研究对象，构建了 lncRNA、miRNA 和 mRNA 的转录组图谱。利用这些数据，我们进行了全转录组分析并构建了 ceRNA 网络。在睾丸中检测到 10 个差异表达的 mRNAs（DEmRNAs）、33 个差异表达的 lncRNAs（DElncRNAs）和 10 个差异表达的 miRNAs（DEmiRNAs），在附睾中检测到 149 个 DEmRNAs、12 个 DElncRNAs 和 10 个 DEmiRNAs。这些基因参与细胞分化和发育，以及 GnRH、MAPK、TGF-β、mTOR、VEGF 和钙离子途径等多种信号通路。随后，我们构建了两个包含 DEmRNAs、DElncRNAs 和 DEmiRNAs 的竞争性内源 RNA（ceRNA）网络。此外，我们鉴定了四个关键的 lncRNA-mRNA-miRNA 相互作用，它们在调控鸡生殖系统中的特定生物学过程中发挥作用：MSTRG.2423.1-gga-miR-1563-PPP3CA 和 MSTRG.10064.2-gga-miR-32-5p-GPR12 调控精子运动；MSTRG.152556.1-gga-miR-9-3p-GREM1/THYN1 调控附睾中的免疫调节；MSTRG.124708.1-gga-miR-375-NDUFB9/YBX1 调控精子成熟和运动。
鸡睾丸和附睾的全转录组测序筛选出了几个关键基因和 ceRNA 调控网络，这些基因可能通过 MAPK、TGF-β、mTOR 和钙离子途径参与附睾免疫、精子发生和精子活力的调控。这些发现有助于我们全面了解公鸡精子发生、成熟和运动的复杂分子过程。
