Birckhead Alice, O'Hare Doig Ryan, Carstens Ann, Jenkins David, Shamsi Shokoofeh
School of Agricultural, Environmental and Veterinary Sciences, Charles Sturt University, Wagga Wagga, Australia.
Preclinical, Imaging and Research Laboratories, South Australian Health and Medical Research Institute (SAHMRI), Adelaide, South Australia, Australia.
Int J Parasitol Parasites Wildl. 2024 Oct 12;25:101002. doi: 10.1016/j.ijppaw.2024.101002. eCollection 2024 Dec.
Micro-computed tomography (micro-CT) is an emerging tool in parasitology that can assist in analysing morphology and host-parasitic interactions. It is a non-destructive, cross-sectional imaging technique that offers good resolution and the ability to create three-dimensional (3D) reconstructions. Here, we used micro-CT to study , which is a zoonotic pentastome parasite that infects dogs and ruminants throughout the world. The aims of this study were to describe the internal and external anatomy of adult specimens using micro-CT, and to describe and compare specimens stained with 0.3% phosphotungstic acid (PTA) and 1% iodine (I). Ten adult specimens were subjected to micro-CT examination. The specimens were fixed in 70% ethanol and stained with 0.3% PTA or 1% I. Both stains offered good tissue contrast. The main identifying external features of (hooks, mouth, buccal cadre) were clearly visible. Virtual sections and 3D reconstructions provided a good overview of the coelomic cavity, with visualisation of the digestive tract, nervous system, and male and female reproductive organs. These micro-CT images and morphological descriptions may serve as an anatomical reference for , in particular, the internal anatomy which has not been described in recent years.
微计算机断层扫描（micro-CT）是寄生虫学中一种新兴的工具，可协助分析形态学以及宿主与寄生虫的相互作用。它是一种非破坏性的横断面成像技术，具有良好的分辨率，并能够创建三维（3D）重建图像。在此，我们使用微CT研究了[具体寄生虫名称未给出]，这是一种人畜共患的舌形虫寄生虫，在全球范围内感染犬类和反刍动物。本研究的目的是使用微CT描述成年[具体寄生虫名称未给出]标本的内部和外部解剖结构，并描述和比较用0.3%磷钨酸（PTA）和1%碘（I）染色的标本。对10个成年[具体寄生虫名称未给出]标本进行了微CT检查。标本用70%乙醇固定，并用0.3% PTA或1% I染色。两种染色都提供了良好的组织对比度。[具体寄生虫名称未给出]的主要外部识别特征（钩、口、颊骨）清晰可见。虚拟切片和3D重建提供了体腔的良好概览，可看到消化道、神经系统以及雄性和雌性生殖器官。这些微CT图像和形态学描述可为[具体寄生虫名称未给出]提供解剖学参考，特别是近年来尚未描述的内部解剖结构。
