School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, Shanxi, China.
Department of Pharmacy, Renmin Hospital, Hubei University of Medicine, Shiyan, 442000, Hubei, China.
Mikrochim Acta. 2024 Nov 5;191(12):728. doi: 10.1007/s00604-024-06801-7.
A visual aptasensor based on the combination of graphitic carbon nitride loaded with platinum nanoparticles (PtNPs/g-CN) and deoxyribonuclease I (DNase I) was developed for detecting acetamiprid. The prepared PtNPs/g-CN exhibited excellent peroxidase (POD)-like activity, demonstrating the capacity to oxidize the colourless o-phenylenediamine (OPD) to produce the coloured product diaminophenazine (DAP) in the presence of hydrogen peroxide (HO). The DNA aptamer of acetamiprid can adsorb onto the surface of PtNPs/g-CN, thereby inhibiting its POD activity. The binding of acetamiprid to its aptamer results in the aptamer desorbing from the surface of PtNPs/g-CN and subsequent digestion by DNase I. Ascribed to the synergistic effect of these factors, the aptasensor can achieve the rapid on-site detection of acetamiprid based on the acetamiprid-induced the colour change of the solution just with the smartphone. Furthermore, due to the remarkable fluorescence signal of DAP at 570 nm, the aptasensor under fluorescence mode enables highly sensitive and quantitative detection of acetamiprid with a linear range of 1 ng/mL to 4 µg/mL and a detection limit as low as 0.31 ng/mL. The aptasensor has successfully realized the detection of acetamiprid in river water and cucumber samples, offering a novel perspective for the rapid assessment of environmental pollution and food safety risks associated with acetamiprid residues.
基于负载铂纳米粒子（PtNPs/g-CN）和脱氧核糖核酸酶 I（DNase I）的石墨相氮化碳的可视化适体传感器被开发用于检测吡虫啉。所制备的 PtNPs/g-CN 表现出优异的过氧化物酶（POD）样活性，能够在存在过氧化氢（HO）的情况下将无色邻苯二胺（OPD）氧化为有色产物二氨基吩嗪（DAP）。吡虫啉的 DNA 适体可以吸附到 PtNPs/g-CN 的表面，从而抑制其 POD 活性。吡虫啉与其适体的结合导致适体从 PtNPs/g-CN 的表面解吸，并随后被 DNase I 消化。由于这些因素的协同作用，该适体传感器可以基于溶液中因吡虫啉引起的颜色变化，利用智能手机快速现场检测吡虫啉。此外，由于 DAP 在 570nm 处具有显著的荧光信号，因此在荧光模式下，该适体传感器能够实现对吡虫啉的高灵敏度和定量检测，线性范围为 1ng/mL 至 4μg/mL，检测限低至 0.31ng/mL。该适体传感器已成功实现了河水中和黄瓜样品中吡虫啉的检测，为快速评估与吡虫啉残留相关的环境污染和食品安全风险提供了新的视角。
