Yu Tengfei, Suo Zhiguang, Zhu Jiaomeng, Xu Yiwei, Ren Wenjie, Liu Yong, Wang Huafen, Wei Min, He Baoshan, Zhao Renyong
College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou, 450001, China.
College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou, 450001, China.
Anal Chim Acta. 2025 Feb 8;1338:343609. doi: 10.1016/j.aca.2025.343609. Epub 2025 Jan 1.
Aflatoxin B1 (AFB1) is a secondary metabolite produced by Aspergillus flavus and Aspergillus parasiticus. This toxin is highly carcinogenic and toxic, posing a serious threat to human and animal health. AFB1 primarily enters the human body through contaminated food, particularly peanuts, corn, nuts, and wheat. These foods are easily contaminated with AFB1 during planting, harvesting, storage, and processing. Therefore, the establishment of an accurate and sensitive method for the detection of AFB1 is essential to safeguard food safety and human health.
This study applied catalytic hairpin assembly (CHA), hybridization chain reaction (HCR), hollow octahedron PtNPs/Pd-CuO bifunctional nanozyme, and AuPtNPs/Ni-Co NCs to develop a colorimetric-electrochemical dual-mode aptasensor for AFB1 detection. Here, Pd-CuO corner-etched octahedra with cavities and oxygen vacancy defects were first designed, followed by the synthesis of PtNPs/Pd-CuO through the loading of PtNPs to enhance the peroxidase-like activity of Pd-CuO. PtNPs/Pd-CuO effectively catalyzed the generation of blue oxidation products from 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (HO). It also enhances the redox reaction of methylene blue (MB) at the electrode and improves the electrochemical signal. AuPtNPs/Ni-Co NCs possess a large specific surface area and excellent conductivity, enhanced the redox signal of MB. Additionally, the aptasensor combined the enzyme-free amplification strategies of CHA and HCR to improve sensitivity. The aptasensor reached limit of detection (LOD) 0.76 pg/mL in electrochemical mode and 0.49 pg/mL in colorimetric mode.
The designed colorimetric-electrochemical dual-mode aptasensor exhibits high selectivity, specificity, stability, and reliability. The dual-mode aptasensor had been successfully applied to the spiked analysis of edible oils, peanuts, and cornmeal, demonstrating excellent recovery rates. Therefore, the dual-mode aptasensor had a wide application prospect in the field of quality supervision and food safety.
黄曲霉毒素B1（AFB1）是由黄曲霉和寄生曲霉产生的次生代谢产物。这种毒素具有高度致癌性和毒性，对人类和动物健康构成严重威胁。AFB1主要通过受污染的食物进入人体，特别是花生、玉米、坚果和小麦。这些食物在种植、收获、储存和加工过程中很容易被AFB1污染。因此，建立一种准确、灵敏的AFB1检测方法对于保障食品安全和人类健康至关重要。
本研究应用催化发夹组装（CHA）、杂交链式反应（HCR）、中空八面体PtNPs/Pd-CuO双功能纳米酶和AuPtNPs/Ni-Co NCs开发了一种用于AFB1检测的比色-电化学双模式适体传感器。在此，首先设计了具有空腔和氧空位缺陷的角蚀刻八面体Pd-CuO，然后通过负载PtNPs合成PtNPs/Pd-CuO以增强Pd-CuO的过氧化物酶样活性。PtNPs/Pd-CuO在过氧化氢（HO）存在下有效地催化3,3',5,5'-四甲基联苯胺（TMB）生成蓝色氧化产物。它还增强了亚甲基蓝（MB）在电极上的氧化还原反应并改善了电化学信号。AuPtNPs/Ni-Co NCs具有大的比表面积和优异的导电性，增强了MB的氧化还原信号。此外，该适体传感器结合了CHA和HCR的无酶扩增策略以提高灵敏度。该适体传感器在电化学模式下的检测限（LOD）为0.76 pg/mL，在比色模式下为0.49 pg/mL。
所设计的比色-电化学双模式适体传感器具有高选择性、特异性、稳定性和可靠性。该双模式适体传感器已成功应用于食用油、花生和玉米粉的加标分析，显示出优异的回收率。因此，该双模式适体传感器在质量监督和食品安全领域具有广阔的应用前景。
