Lambert W E, De Leenheer A P, Lefevere M F
J Chromatogr Sci. 1986 Feb;24(2):76-9. doi: 10.1093/chromsci/24.2.76.
A new, sensitive and selective detection method for vitamin K1 in human serum is reported. After the chromatographic separation, vitamin K is converted to its hydroquinone form in a wet-chemical post-column reduction reaction with tetramethylammonium octahydridotriborate. The reaction proceeds in an open tubular knitted reaction coil at elevated temperature. This system, when combined with a two-step multidimensional liquid chromatographic separation, has proven useful for the quantitative determination of serum concentrations of vitamin K1 in normal, healthy individuals.
报道了一种用于检测人血清中维生素K1的新型、灵敏且具选择性的检测方法。色谱分离后，维生素K在与八氢三硼酸四甲铵的湿化学柱后还原反应中转化为对苯二酚形式。该反应在高温下于开放式管状编织反应盘管中进行。该系统与两步多维液相色谱分离相结合，已证明可用于定量测定正常健康个体血清中维生素K1的浓度。
