Division of Molecular Centre of Excellence in Molecular Biology (CEMB), University of the Punjab, 87 West Canal Bank Road, Thokar Niaz baig, ، Quaid-i-Azam Campus, Lahore, 53700, Punjab, Pakistan.
Arch Virol. 2024 Nov 5;169(11):239. doi: 10.1007/s00705-024-06163-8.
Hepatitis B virus (HBV) infection is a major public health issue and is responsible for considerable morbidity and mortality globally. In Pakistan, the prevalence of chronic HBV infection varies from 2% to 4%, with an estimated exposure rate of approximately 34%. The major objective of this study was to determine the prevalence of HBV genotypes and the pattern of escape mutations in the HBV S gene in two major provinces of Pakistan: Punjab and Khyber Pakhtunkhwa. A total of 146 serum/plasma samples collected from hepatitis B patients were sequenced by the Sanger method. Phylogenetic analysis indicated the intermixed circulation of closely related strains of HBV genotype D and subgenotypes HBV/D1, HBV/D2, and HBV/A2 in both provinces, and various escape mutations (Y100C, P120S/T, G119R, C121S/Y, R122K, T126I, A128V, Q129H/R, G130R, T131N/I, M133T/I, Y134N, C137W, S143L, and D144E) were identified. These findings provide important insights into the current prevalence of HBV genotypes in Pakistan and will help in the establishment of more-efficient disease interventions and patient management strategies.
乙型肝炎病毒 (HBV) 感染是一个主要的公共卫生问题，在全球范围内造成了相当大的发病率和死亡率。在巴基斯坦，慢性 HBV 感染的流行率在 2%到 4%之间，估计暴露率约为 34%。本研究的主要目的是确定巴基斯坦两个主要省份旁遮普省和开伯尔-普赫图赫瓦省 HBV 基因型的流行率和 HBV S 基因逃逸突变的模式。共对来自乙型肝炎患者的 146 份血清/血浆样本进行了 Sanger 测序。系统发育分析表明，HBV 基因型 D 及其亚基因型 HBV/D1、HBV/D2 和 HBV/A2 的密切相关株在这两个省混合传播，并存在各种逃逸突变（Y100C、P120S/T、G119R、C121S/Y、R122K、T126I、A128V、Q129H/R、G130R、T131N/I、M133T/I、Y134N、C137W、S143L 和 D144E）。这些发现为了解巴基斯坦目前 HBV 基因型的流行情况提供了重要线索，并有助于制定更有效的疾病干预和患者管理策略。
