Guo Xiaoyan, Yu Hongyan, Wang Xiansheng, Zhao Shifeng, Wang Chunyan, Wang Shuai
Pulmonary and Critical Care Medicine II, Affiliated Hospital of Hebei University of Engineering, Handan, 056002, Hebei, China.
Department of Function I, Affiliated Hospital of Hebei University of Engineering, No. 81 Congtai Road, Congtai District, Handan, 056002, Hebei, China.
Mol Biotechnol. 2024 Nov 5. doi: 10.1007/s12033-024-01306-3.
Non-small cell lung cancer (NSCLC), including squamous cell carcinoma and adenocarcinoma, ranks among the top 10 cancers worldwide in terms of prevalence and mortality. NSCLC, a highly malignant tumor, exhibits distant invasion and migration as well as an unfavorable prognosis. As an innovative circular RNA, hsa _circ_0109320 (circ_0109320) has been recognized as a promising cancer modulator. However, our understanding of the influence of circ_0109320 in NSCLC remains insufficient. Our research explored the clinical significance and effects of circ_0109320 on oncogenic non-small cell lung cancer (NSCLC) phenotypes. Microarray analysis and qPCR indicated that circ_0109320 expression in NSCLC specimens increased relative to that in adjacent normal tissues and was further elevated in metastatic lymph nodes. The specimens acquired from 25 patients confirmed these findings. Additionally, circ_0109320 indicated a good score (AUC = 0.688, P = 0.013) on the ROC curves, which suggests its suitability as a promising biomarker for lung cancer. Meanwhile, circ_0109320 was noticeably upregulated in lung cancer (LC) cell lines compared to human bronchial epithelial cells. Next, we performed loss- and gain-of-function experiments to examine the role of circ_0109320 in the tumor phenotypes of the cell lines. We observed that depletion or overexpression of circ_0109320 did not alter cell viability. However, the ectopic removal of circ_0109320 repressed the migration and invasion of A549 and SK-MES-1 cells, whereas circ_0109320 overexpression promoted cell migration and invasion. Furthermore, the examination of epithelial-mesenchymal transition (EMT) markers indicated that circ_0109320 elevates cell EMT activity. In conclusion, circ_0109320 level was highly associated with increased tumor cell proliferation and metastasis. circ_0109320 could be a promising predictor of clinical outcomes and a reliable target to treat NSCLC by inhibiting metastasis.
非小细胞肺癌（NSCLC），包括鳞状细胞癌和腺癌，在全球癌症的患病率和死亡率方面位列前十。NSCLC是一种高度恶性肿瘤，具有远处侵袭和转移能力，预后不佳。作为一种新型环状RNA，hsa_circ_0109320（circ_0109320）已被认为是一种有前景的癌症调节因子。然而，我们对circ_0109320在NSCLC中的影响仍了解不足。我们的研究探讨了circ_0109320对致癌性非小细胞肺癌（NSCLC）表型的临床意义和影响。微阵列分析和qPCR表明，NSCLC标本中circ_0109320的表达相对于相邻正常组织有所增加，并且在转移淋巴结中进一步升高。从25例患者获取的标本证实了这些发现。此外，circ_0109320在ROC曲线上显示出良好的评分（AUC = 0.688，P = 0.013），这表明它适合作为肺癌的一种有前景的生物标志物。同时，与人类支气管上皮细胞相比，circ_0109320在肺癌（LC）细胞系中明显上调。接下来，我们进行了功能丧失和功能获得实验，以研究circ_0109320在细胞系肿瘤表型中的作用。我们观察到circ_0109320的缺失或过表达并未改变细胞活力。然而，circ_0109320的异位去除抑制了A549和SK-MES-1细胞的迁移和侵袭，而circ_0109320的过表达促进了细胞迁移和侵袭。此外，上皮-间质转化（EMT）标志物的检测表明，circ_0109320提高了细胞的EMT活性。总之，circ_0109320水平与肿瘤细胞增殖和转移增加高度相关。circ_0109320可能是临床结果的一个有前景的预测指标，并且是通过抑制转移来治疗NSCLC的一个可靠靶点。
