State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China.
Institute for Chemical Carcinogenesis, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China.
Mol Cancer. 2020 Jun 3;19(1):101. doi: 10.1186/s12943-020-01221-6.
Lung cancer has high morbidity and mortality worldwide with non-small cell lung cancer (NSCLC) accounting for 85% of the cases. Therapies for lung cancer have relatively poor outcomes and further improvements are required. Circular RNAs have been reported to participate in the occurrence and progression of cancer. Information on the functions and mechanism of circRNAs in lung cancer is limited and needs more exploration.
We detected expression of genes and proteins by qPCR and western blot. Function of circSATB2 was investigated using RNA interference and overexpression assays. Location of circSATB2 was assessed by fluorescence in situ hybridization (FISH). Interaction of circSATB2, miR-326 and FSCN1 was confirmed by dual-luciferase reporter assay.
Data from the investigation showed that circSATB2 was highly expressed in NSCLC cells and tissues. circSATB2 positively regulated fascin homolog 1, actin-bundling protein 1 (FSCN1) expression via miR-326 in lung cancer cells. Furthermore, circSATB2 can be transferred by exosomes and promote the proliferation, migration and invasion of NSCLC cells, as well as induce abnormal proliferation in normal human bronchial epithelial cells. Also, circSATB2 was highly expressed in serumal exosomes from lung cancer patients with high sensitivity and specificity for clinical detection and was related to lung cancer metastasis.
circSATB2 participated in the progression of NSCLC and was differentially expressed in lung cancer tissue and serumal exosomes. circSATB2 may be potential biomarker for the diagnosis of NSCLC.
肺癌在全球范围内具有较高的发病率和死亡率，其中非小细胞肺癌（NSCLC）占 85%。肺癌的治疗方法效果相对较差，需要进一步改善。环状 RNA 已被报道参与癌症的发生和进展。关于环状 RNA 在肺癌中的功能和机制的信息有限，需要更多的探索。
我们通过 qPCR 和 Western blot 检测基因和蛋白质的表达。通过 RNA 干扰和过表达实验研究 circSATB2 的功能。通过荧光原位杂交（FISH）评估 circSATB2 的位置。通过双荧光素酶报告实验证实 circSATB2、miR-326 和 FSCN1 的相互作用。
研究数据表明，circSATB2 在 NSCLC 细胞和组织中高表达。circSATB2 通过 miR-326 正向调节 fascin 同源物 1、肌动蛋白结合蛋白 1（FSCN1）在肺癌细胞中的表达。此外，circSATB2 可以通过外泌体转移，并促进 NSCLC 细胞的增殖、迁移和侵袭，同时诱导正常人支气管上皮细胞异常增殖。此外，circSATB2 在肺癌患者血清外泌体中高表达，具有较高的临床检测灵敏度和特异性，与肺癌转移有关。
circSATB2 参与了 NSCLC 的进展，在肺癌组织和血清外泌体中表达差异。circSATB2 可能是 NSCLC 诊断的潜在生物标志物。
