Fu Jiafang, Zhang Peipei, Yin Xunzhe, Zhu Lingjia, Zong Gongli, Zhong Chuanqing, Cao Guangxiang
Biomedical Sciences College & Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji'nan, China.
School of Municipal and Environmental Engineering, Shandong Jianzhu University, Ji'nan, China.
Folia Microbiol (Praha). 2024 Nov 5. doi: 10.1007/s12223-024-01219-3.
Worldwide prevalence of multi-antibiotic resistant bacteria is rapidly increasing, and the education of undergraduates and graduates about antibiotic resistance and its associated horizontal gene transfer is critical in the general effort to confront the spread of antibiotic resistance. In this study, a deeper understanding of antibiotic resistance and horizontal gene transfer was achieved by biomedical undergraduate students through a scientific research programme. The enthusiasm of students to participate in the training programme was very high, and results revealed that each student could identify the antibiotic resistance integrative and conjugative element from the Stenotrophomonas maltophilia MER1 genome. Each student could also draw the phylogenetic relationship of the antibiotic resistance integrative and conjugative element. In addition, students proved the horizontal transfer of antibiotic resistance genes from S. maltophilia MER1 to Escherichia coli strain 25DN through conjugation and PCR assays. Each group of students was able to obtain the expected results, indicating that the outcome of the scientific research programme was highly reproducible. This programme improved the theoretical knowledge about antibiotic resistance and horizontal gene transfer and the research skills of biomedical sciences students. Through this programme, students learned that antibiotic resistance genes can be horizontally transferred among different bacteria, laying a solid foundation for students to value the importance of the appropriate use of antibiotics in their future work and life.
全球多重耐药菌的流行率正在迅速上升，在应对抗生素耐药性传播的总体努力中，对本科生和研究生进行抗生素耐药性及其相关水平基因转移的教育至关重要。在本研究中，生物医学专业的本科生通过一个科研项目对抗生素耐药性和水平基因转移有了更深入的了解。学生参与培训项目的积极性很高，结果显示每个学生都能从嗜麦芽窄食单胞菌MER1基因组中识别出抗生素耐药整合和接合元件。每个学生还能绘制出抗生素耐药整合和接合元件的系统发育关系。此外，学生们通过接合和PCR检测证明了抗生素耐药基因从嗜麦芽窄食单胞菌MER1水平转移到大肠杆菌菌株25DN。每组学生都能获得预期结果，表明该科研项目的结果具有高度可重复性。这个项目提高了生物医学专业学生关于抗生素耐药性和水平基因转移的理论知识以及研究技能。通过这个项目，学生们了解到抗生素耐药基因可以在不同细菌之间水平转移，为学生在未来工作和生活中重视合理使用抗生素的重要性奠定了坚实基础。
