Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan.
Methods Mol Biol. 2025;2869:15-20. doi: 10.1007/978-1-0716-4204-7_3.
RNA extraction is a critical step in gene expression analysis. While numerous RNA extraction kits are commercially available, most kits cannot be utilized for RNA extraction from rice endosperm that contains abundant starch. Here, I describe a three-step RNA extraction from rice starchy endosperm. The process involves phenol/chloroform extraction, ethanol precipitation, and lithium chloride precipitation. This method yields an abundant and high-quality total RNA even from a single starchy endosperm.
RNA 提取是基因表达分析的关键步骤。虽然有许多 RNA 提取试剂盒在商业上可用，但大多数试剂盒不能用于从富含淀粉的水稻胚乳中提取 RNA。在这里，我描述了从水稻淀粉胚乳中进行三步法 RNA 提取的方法。该过程涉及酚/氯仿提取、乙醇沉淀和氯化锂沉淀。即使仅从单个淀粉胚乳中，该方法也能获得丰富且高质量的总 RNA。
