Dos Santos João Victor Batista, Dos Santos Anna Luiza Hora, Alves André Mota, de Oliveira Matheus Resende, de Medeiros Gomes Simplicio Kalina Maria, Silva Lorena Maciel Santos, de Jesus João Victor, Soares Michelle Evangelista, da Costa Ana Cinthia Santos, da Silva Weslania Sousa Inacio, de Melo Cláudia Moura, Madi Rubens Riscala, Lima Victor Fernando Santana
Department of Veterinary Medicine, Federal University of Sergipe, Nossa Senhora da Glória, SE, Brazil.
Laboratory of Animal Parasitic Diseases, Federal University of Sergipe, Nossa Senhora da Glória, SE, Brazil.
Acta Parasitol. 2024 Dec;69(4):2055-2063. doi: 10.1007/s11686-024-00935-7. Epub 2024 Nov 5.
This study aimed to assess the influence of the presence of synanthropic flies in food preparation environments on the transmission of potentially zoonotic gastrointestinal protozoa.
Flies were captured using a glass containing water, fruits, and pieces of protein.
Approximately 260 flies from four different species were captured: Musca domestica (76.92%; 200/260), Cochliomyia hominivorax (17.31%; 45/260), Lucilia cuprina (3.85%; 10/260), and Hydrotaea aenescens (1.92%; 5/260). Protozoa were identified through microscopic analysis after macerating these arthropods contained in their respective eppendorfs, using two techniques, namely Mini-FLOTAC and centrifugal sedimentation with Ziehl-Neelsen staining. The analysis revealed a concerning scenario regarding the epidemiological chain of gastrointestinal protozoa in Sergipe, Northeast Brazil. The positivity rate was 26.92% (14/52) of the pools, with 100.00% (5/5) in Aracaju, 30.00% (3/10) in Nossa Senhora do Socorro, 27.27% (3/11) in Nossa Senhora da Glória, 12.50% (2/16) in Carmópolis, and 10.00% (1/10) in Nossa Senhora das Dores. Two species of protozoa were identified, namely Cryptosporidium spp. (23.08%; 12/52) and Entamoeba spp. (9.62%; 5/52). The involvement of two fly species in the maintenance of the life cycle of these protozoa was noticeable, specifically M. domestica (27.50%; 11/40) and C. hominivorax (22.22%; 2/9).
Counties analyzed had an human development index (HDI) considered medium; however, they still faced socioeconomic problems such as absence of sanitation systems, waste accumulation in the streets, and illegal waste disposal, which could favor the proliferation of these vectors and the spread of these gastrointestinal protozoa.
本研究旨在评估食源苍蝇在食物制备环境中的存在对潜在人畜共患胃肠道原生动物传播的影响。
使用装有水、水果和蛋白质块的玻璃杯捕获苍蝇。
共捕获了来自四个不同物种的约260只苍蝇：家蝇（76.92%；200/260）、嗜人瘤蝇（17.31%；45/260）、铜绿蝇（3.85%；10/260）和铜绿腐蝇（1.92%；5/260）。在各自的微量离心管中对这些节肢动物进行浸软处理后，通过显微镜分析，采用两种技术即Mini-FLOTAC和齐尔-尼尔森染色离心沉淀法鉴定原生动物。分析揭示了巴西东北部塞尔希培州胃肠道原生动物流行病学链的令人担忧的情况。混合样本的阳性率为26.92%（14/52），其中阿拉卡茹为100.00%（5/5），索科罗圣母镇为30.00%（3/10），荣耀圣母镇为27.27%（3/11），卡尔莫波利斯为12.50%（2/16），痛苦圣母镇为10.00%（1/10）。鉴定出两种原生动物，即隐孢子虫属（23.08%；12/52）和内阿米巴属（9.62%；5/52）。值得注意的是，两种苍蝇参与了这些原生动物生命周期的维持，特别是家蝇（27.50%；11/40）和嗜人瘤蝇（22.22%；2/9）。
所分析的县人类发展指数（HDI）为中等水平；然而，它们仍然面临社会经济问题，如缺乏卫生系统、街道垃圾堆积和非法垃圾处理，这可能有利于这些病媒的繁殖以及这些胃肠道原生动物的传播。
