Taleahmad Fatemeh, Khalili Mohsen, Haddadzadeh-Niri Narges, Joneidi Ensyie, Taleahmad Sara, Roghani Mehrdad
Department of Physiology, School of Medicine, Shahed University, Tehran, Iran.
School of Basic Sciences, Shahed University, Tehran, Iran.
Reprod Biol. 2024 Dec;24(4):100966. doi: 10.1016/j.repbio.2024.100966. Epub 2024 Nov 4.
This study evaluated diosgenin effects on methotrexate-induced testicular injury in the rats. A single dose of methotrexate (MTX) (20 mg/kg, i.p) was administered, followed by two weeks of diosgenin treatment via gavage starting one day before methotrexate injection. Testicular damage was evaluated through histological examination of seminiferous tubules, as well as analysis of serum testosterone level, oxidative stress and inflammation biomarkers, and antioxidant levels. The results of this study showed that in the MTX-exposed group, oxidative stress indices of malondialdehyde (MDA), reactive oxygen species (ROS), nitrite and indices of inflammation consisting of tumor necrosis factor α (TNFα), and interleukin 6 (IL-6) have a significant increase compared to the control group. Additionally, reductions were observed in antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH). In addition, testosterone level decreased and signs of testicular damage were observed in the MTX group. Conversely, in the group treated with diosgenin alongside MTX at a dosage of 50 mg/kg, there was a significant decrease in oxidative stress markers (MDA, ROS, nitrite) and inflammatory markers (TNFα and IL-6). Moreover, there was a significant increase in the levels of antioxidant enzymes (SOD, CAT, and GSH). Diosgenin appears to have the potential to protect testicular tissue from damage caused by the toxic effects of MTX through the reduction of oxidative stress and inflammation.
本研究评估了薯蓣皂苷元对甲氨蝶呤诱导的大鼠睾丸损伤的影响。给予单剂量甲氨蝶呤（MTX）（20mg/kg，腹腔注射），然后在甲氨蝶呤注射前一天开始通过灌胃给予两周的薯蓣皂苷元治疗。通过对生精小管进行组织学检查，以及分析血清睾酮水平、氧化应激和炎症生物标志物以及抗氧化剂水平来评估睾丸损伤。本研究结果表明，与对照组相比，在MTX暴露组中，丙二醛（MDA）、活性氧（ROS）、亚硝酸盐等氧化应激指标以及由肿瘤坏死因子α（TNFα）和白细胞介素6（IL-6）组成的炎症指标均显著升高。此外，观察到抗氧化酶包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽（GSH）减少。另外，MTX组睾酮水平降低，并观察到睾丸损伤迹象。相反，在以50mg/kg剂量与MTX一起用薯蓣皂苷元治疗的组中，氧化应激标志物（MDA、ROS、亚硝酸盐）和炎症标志物（TNFα和IL-6）显著降低。此外，抗氧化酶（SOD、CAT和GSH）水平显著升高。薯蓣皂苷元似乎有可能通过降低氧化应激和炎症来保护睾丸组织免受MTX毒性作用造成的损伤。
