Department of Nuclear Medicine, Manipal College of Health Professions, Manipal Academy of Higher Education, Manipal, India.
Department of Cell and Molecular Biology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India.
Photodermatol Photoimmunol Photomed. 2024 Nov;40(6):e13010. doi: 10.1111/phpp.13010.
Mitochondria could be an important target for ultraviolet radiation (UVR)-induced skin damage. Therefore, protecting mitochondria using mitochondria-targeted antioxidants may protect skin from UV-induced photodamage.
In the present study, UVR-induced skin damage model was developed by irradiating male Wistar rats with UVB at a dose of 120 mJ/cm, twice a week for a period of 5 weeks. Mito-TEMPO was administered intraperitoneally (0.1 mg/kg b.w.) twice a week for 5 weeks. After 5 weeks of treatment period, animals were sacrificed and the dorsal skin tissues were collected. Physical examinations of the skin for analyzing wrinkle formation and epidermal thickening were carried out. Skin tissues were used for the evaluation of histopathological changes, mitochondrial dysfunction analysis, and mitochondrial membrane potential.
Physical and histological examination showed that mito-TEMPO protected from the damaging effect of UVB radiation. A significant increase in mitochondrial reactive oxygen species (mtROS) generation with a concomitant increase in mitochondrial lipid peroxidation (mtLPO) was observed in UV-irradiated groups. UV-induced generation of mtROS and mtLPO formation was effectively reduced by mito-TEMPO. Mito-TEMPO pre-treatment improved mitochondrial complex II activity, which was significantly reduced in the UV-irradiated group.
The results suggested that mito-TEMPO effectively protected skin tissue against UV-induced oxidative stress and damage.
线粒体可能是紫外线（UVR）诱导皮肤损伤的一个重要靶点。因此，使用靶向线粒体的抗氧化剂来保护线粒体可能有助于防止皮肤受到 UVR 诱导的光损伤。
在本研究中，通过以 120mJ/cm 的剂量对雄性 Wistar 大鼠每周两次照射 UVB 来建立 UVR 诱导的皮肤损伤模型，持续 5 周。每周两次腹膜内给予 mito-TEMPO（0.1mg/kg b.w.），共 5 周。治疗 5 周后，处死动物并收集背部皮肤组织。进行皮肤褶皱形成和表皮增厚的物理检查，以分析皮肤状况。评估皮肤组织的组织病理学变化、线粒体功能障碍分析和线粒体膜电位。
物理和组织学检查表明，mito-TEMPO 可防止 UVB 辐射的损伤作用。在 UV 照射组中，观察到线粒体活性氧（mtROS）生成显著增加，同时线粒体脂质过氧化（mtLPO）增加。mito-TEMPO 可有效减少 UV 诱导的 mtROS 生成和 mtLPO 形成。mito-TEMPO 预处理可改善线粒体复合物 II 活性，而该活性在 UV 照射组中显著降低。
结果表明，mito-TEMPO 可有效保护皮肤组织免受 UVR 诱导的氧化应激和损伤。
