Rabey Haddad A El, Rezk Samar M, Abusaber Aseel, Khlabi Rwaah, Alhawiti Ayah H, M Algorayed Romana, Bakry Nadia
Biochemistry Department, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia.
Clinical Nutrition Department, Mahalla Hepatology Teaching Hospital, Ministry of Health and Population, Gharbyia, El-Mahalla El-Kubra, Egypt.
Int J Nephrol. 2024 Oct 28;2024:8023989. doi: 10.1155/2024/8023989. eCollection 2024.
The purpose of this study was to test the protective effect of (WS) against the harmful effects of mercuric chloride (HgCl)-induced kidney failure at the histological, biochemical, and immune levels in Wistar rats. The study assessed the biochemical and immunological changes in five groups ( = 6): Group 1 (G1) was the negative control, and the other rats received a single subcutaneous dose of HgCl (2.5 mg/kg in 0.5 mL of 0.9% saline solution) and randomly divided into 4 groups. Group 2 (G2) was the positive control and left without treatment. Groups 3, 4, and 5 (G3, G4, and G5) were treated with different doses of WS root powder for 30 days. The HgCl-positive group showed significant signs of renal toxicity as reflected by increased levels of kidney function parameters (blood urea nitrogen, urea, and creatinine), inflammatory biomarkers, immunological indices (SDF-1, IL-6, NGAL, and KIM-1), and oxidative stress (SOD, TAC, CAT, GSH, and MDA). The positive group rats also showed drastic pathological changes in renal tissues. Different doses of WS treatment significantly reduced the levels of all biochemical markers and decreased pathological damage to the kidney tissues. The antioxidant, phenolic, and flavonoid constituents of WS root powder helped protect rats' kidneys against HgCl-induced kidney toxicity in male rats.
本研究的目的是在组织学、生化和免疫水平上，测试（WS）对氯化汞（HgCl）诱导的Wistar大鼠肾衰竭有害影响的保护作用。该研究评估了五组（每组n = 6）的生化和免疫学变化：第1组（G1）为阴性对照，其他大鼠接受单次皮下注射HgCl（2.5 mg/kg，溶于0.5 mL 0.9%盐溶液），并随机分为4组。第2组（G2）为阳性对照，不进行治疗。第3、4和5组（G3、G4和G5）用不同剂量的WS根粉治疗30天。HgCl阳性组表现出明显的肾毒性迹象，表现为肾功能参数（血尿素氮、尿素和肌酐）、炎症生物标志物、免疫指标（SDF-1、IL-6、NGAL和KIM-1）以及氧化应激（SOD、TAC、CAT、GSH和MDA）水平升高。阳性组大鼠的肾组织也出现了严重的病理变化。不同剂量的WS治疗显著降低了所有生化标志物的水平，并减少了肾组织的病理损伤。WS根粉中的抗氧化剂、酚类和黄酮类成分有助于保护雄性大鼠的肾脏免受HgCl诱导的肾毒性。
