Okecha Tonny, Abila Derrick B, Nabbale Dorothy L, Katongole Fauz, Yahaya James J, Lukande Robert, Kalungi Sam, Nalwoga Hawa
Department of Pathology, Uganda Cancer Institute, Kampala, Uganda.
Department of Pathology, Uganda Christian University, Kampala, Uganda.
Obstet Gynecol Int. 2024 Oct 29;2024:9527113. doi: 10.1155/2024/9527113. eCollection 2024.
gene dysfunction seen in epithelial ovarian carcinomas often results from germline mutations, somatic mutations, and promoter methylation. Identification of tumors with loss of protein expression has shown to have therapeutic and prognostic implications. The aim of this study was to determine the expression of protein in epithelial ovarian cancer (EOC) and the associated clinicopathological characteristics. This was a cross-sectional laboratory-based study that used paraffin-embedded tissue blocks of patients histologically diagnosed with EOC from January 2010 to August 2018. Tissue sections were stained with hematoxylin and eosin (H&E) for histological confirmation and with immunohistochemistry (IHC) using a mouse-derived monoclonal antibody MS110 for protein expression. The association between protein expression and independent variables was determined using Pearson's Chi-square test. A total of 104 tissue blocks from patients with EOC were included in the study with a mean age of 48.7 ± 12.8 years. Serous tumors were the most common which comprised 74.0% (77/104) of all the tumors and majority of them 75.3% (58/77) were high grade. Loss of expression of protein expression was found in 33.7% (33/98) of all the cases. There was no statistically significant association between expression and age of patients, tumor grade, and histological subtype. There is a high expression of altered expression in tissues of EOC. Although it has not shown association with age of patients, histology types, and tumor grade, further studies need to assess its influence of the survival of cancer patients with EOC.
上皮性卵巢癌中出现的基因功能障碍通常源于种系突变、体细胞突变和启动子甲基化。鉴定蛋白表达缺失的肿瘤已显示出具有治疗和预后意义。本研究的目的是确定蛋白在上皮性卵巢癌（EOC）中的表达情况以及相关的临床病理特征。这是一项基于实验室的横断面研究，使用了2010年1月至2018年8月间经组织学诊断为EOC的患者的石蜡包埋组织块。组织切片用苏木精和伊红（H&E）染色以进行组织学确认，并用鼠源单克隆抗体MS110进行免疫组织化学（IHC）检测蛋白表达。使用Pearson卡方检验确定蛋白表达与自变量之间的关联。本研究共纳入了104例EOC患者的组织块，平均年龄为48.7±12.8岁。浆液性肿瘤最为常见，占所有肿瘤的74.0%（77/104），其中大多数75.3%（58/77）为高级别。在所有病例的33.7%（33/98）中发现蛋白表达缺失。蛋白表达与患者年龄、肿瘤分级和组织学亚型之间无统计学显著关联。EOC组织中存在改变的蛋白高表达。尽管它与患者年龄、组织学类型和肿瘤分级未显示出关联，但进一步的研究需要评估其对EOC癌症患者生存的影响。
