Zhang Lipeng, Teng Yuanxu, Song Yue, Li Junpeng, Zhang Zhen, Xu Yuanyuan, Fan Dongying, Wang Lujia, Ren Yi, He Juan, Song Shiren, Xi Xiaojun, Liu Huaifeng, Ma Chao
Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization of Xinjiang Production and Construction Corps, Department of Horticulture, Agricultural College of Shihezi University, Shihezi, China.
Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Front Plant Sci. 2024 Oct 22;15:1484892. doi: 10.3389/fpls.2024.1484892. eCollection 2024.
With global warming, heat stress has been recognized as a significant factor limiting grapevine development and fruit quality. MicroRNAs (miRNAs) are a class of small non-coding RNAs known to play crucial regulatory roles in stress resistance. Hence, there is an immediate requirement to cultivate and identify grapevine varieties that are resistant to heat and explore miRNA-mediated heat stress defense mechanisms. In this study, we assessed the thermal resistance of 38 grape germplasm resources and identified a series of miRNAs involved in heat stress resistance. The CK (25°C) and HS (45°C) groups of "Shenyue" cuttings of grapes were used as experimental materials for next-generation sequencing and construct libraries of small RNAs. A total of 177 known and 20 novel miRNAs were detected in the libraries. Differential expression analysis identified 65 differentially expressed miRNAs (DEMs) using the DE-Seq procedure. Furthermore, RT-qPCR validation confirmed complementary expression profiles of eight DEMs and their target genes between the HS and CK groups. Heterologous transformation further identified the function of Vvi-miR3633a downregulated under heat stress in . In the heterologous expression lines, the survival rate was reduced by high temperature treatment indicating the ability of Vvi-miR3633a to regulate heat resistance. Assessing the heat resistance of grape species and the expression patterns of miRNA in response to high temperatures may reveal the molecular processes of heat resistance regulation mediated by miRNA in grapes under heat stress.
随着全球变暖，热胁迫已被认为是限制葡萄生长发育和果实品质的重要因素。微小RNA（miRNA）是一类小的非编码RNA，已知在抗逆性中发挥关键的调控作用。因此，迫切需要培育和鉴定耐热葡萄品种，并探索miRNA介导的热胁迫防御机制。在本研究中，我们评估了38份葡萄种质资源的耐热性，并鉴定了一系列参与耐热性的miRNA。以葡萄“神月”插条的CK（25°C）和HS（45°C）组为实验材料进行下一代测序，并构建小RNA文库。文库中总共检测到177个已知miRNA和20个新的miRNA。差异表达分析使用DE-Seq程序鉴定出65个差异表达的miRNA（DEM）。此外，RT-qPCR验证证实了8个DEM及其靶基因在HS组和CK组之间的互补表达谱。异源转化进一步鉴定了热胁迫下下调的Vvi-miR3633a在[具体植物名称未给出]中的功能。在异源表达系中，高温处理降低了存活率，表明Vvi-miR3633a具有调控耐热性的能力。评估葡萄品种的耐热性以及miRNA响应高温的表达模式，可能揭示热胁迫下葡萄中miRNA介导的耐热性调控的分子过程。
