Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271000, China.
Department of Horticulture, College of Agriculture, Shihezi University, Shihezi 832003, Xinjiang, China; Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Shihezi 832003, Xinjiang, China.
Plant Sci. 2022 Dec;325:111450. doi: 10.1016/j.plantsci.2022.111450. Epub 2022 Sep 6.
As a kind of small molecular weight proteins, many peptides have been discovered, including peptides encoded by pri-miRNA (miPEPs). Similar as traditional phytohormone or signaling molecular, these peptides participate in numerous plant growth processes. MicroRNAs (miRNAs) play an important regulatory role in plant stress response. While the roles of miPEPs in response to abiotic stress has not been studied now. In this study, to explore whether miPEPs could contribute to low temperature (4ºC) tolerance of plants, the expression pattern of 23 different vvi-MIRs were analyzed by qRT-PCR in 'Thompson Seedless' (Vitis vinifera) plantlets under cold stress (4ºC) firstly, and vvi-MIR172b and vvi-MIR3635b which showed an elevated expression levels were selected to identify miPEPs. Through transient expression, one small open reading frame (sORF) in each of the two pri-miRNAs could increase the expression of corresponding vvi-MIR, and the amino acid sequences of sORFs were named vvi-miPEP172b and vvi-miPEP3635b, respectively. The synthetic vvi-miPEP172b and vvi-miPEP3635b were applied to the grape plantlets, and the tissue culture plantlets exhibited a higher cold tolerance compared with the control groups. These results revealed the effective roles of miPEPs in plant cold stress resistance for the first time, providing a theoretical basis for the future application of miPEPs to agricultural production.
作为一种小分子蛋白质，已经发现了许多肽，包括 pri-miRNA（miPEPs）编码的肽。与传统的植物激素或信号分子相似，这些肽参与了许多植物生长过程。microRNAs (miRNAs) 在植物应激反应中发挥着重要的调节作用。然而，目前还没有研究 miPEPs 在应对非生物胁迫中的作用。在这项研究中，为了探讨 miPEPs 是否有助于植物对低温（4°C）的耐受，首先通过 qRT-PCR 分析了‘Thompson Seedless’（Vitis vinifera）幼苗中 23 种不同 vvi-MIRs 在冷胁迫（4°C）下的表达模式，然后选择表达水平升高的 vvi-MIR172b 和 vvi-MIR3635b 来鉴定 miPEPs。通过瞬时表达，两种 pri-miRNAs 中的每个都可以增加相应的 vvi-MIR 的表达，并且 sORFs 的氨基酸序列分别命名为 vvi-miPEP172b 和 vvi-miPEP3635b。合成的 vvi-miPEP172b 和 vvi-miPEP3635b 被应用于葡萄幼苗，与对照组相比，组织培养的幼苗表现出更高的耐寒性。这些结果首次揭示了 miPEPs 在植物抗冷胁迫中的有效作用，为未来将 miPEPs 应用于农业生产提供了理论依据。
