无菌宿主酵母（SHY）：用于重组DNA实验的真核生物遏制系统。

Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.

作者信息

Botstein D, Falco S C, Stewart S E, Brennan M, Scherer S, Stinchcomb D T, Struhl K, Davis R W

出版信息

Gene. 1979 Dec;8(1):17-24. doi: 10.1016/0378-1119(79)90004-0.

Abstract

A system of biological containment for recombinant DNA experiments in Saccharomyces cerevisiae (Brewer's/Baker's yeast) is described. The principle of containment is sterility: the haploid host strains all contain a mating-type-non-specific sterile mutation. The hosts also contain four auxotrophic mutations suitable for selection for the various kinds of vectors used. All vectors are derivatives of pBR322 which can be selected and maintained in both yeast and Escherichia coli. The system has recently been certified at the HV2 level by the National Institutes of Health.

摘要

本文描述了一种用于酿酒酵母（啤酒酵母/面包酵母）中重组DNA实验的生物遏制系统。遏制的原理是不育性：单倍体宿主菌株均含有一种交配型非特异性不育突变。宿主还含有四种适合用于选择各种所用载体的营养缺陷型突变。所有载体均为pBR322的衍生物，可在酵母和大肠杆菌中进行选择和维持。该系统最近已获得美国国立卫生研究院的HV2级认证。