带有酶切位点文库的载体。III. 大肠杆菌-酿酒酵母穿梭载体。
Vectors with restriction-site banks. III. Escherichia coli-Saccharomyces cerevisiae shuttle vectors.
作者信息
Heusterspreute M, Oberto J, Vinh H T, Davison J
出版信息
Gene. 1985;34(2-3):363-6. doi: 10.1016/0378-1119(85)90146-5.
PMID:2989114
Abstract
The bank of unique restriction sites present in plasmid pJRD158 has been incorporated into new vectors carrying selective markers and replicons derived from commonly used Escherichia coli-Saccharomyces cerevisiae shuttle vectors pJDB207 and YRp7. The new vectors pMH158 and pJO158 have 21 and 23 unique restriction sites, respectively, and their complete DNA sequences are known.
摘要
质粒pJRD158中存在的独特限制酶切位点库已被整合到新载体中，这些新载体携带了源自常用大肠杆菌-酿酒酵母穿梭载体pJDB207和YRp7的选择标记和复制子。新载体pMH158和pJO158分别有21个和23个独特的限制酶切位点，并且它们的完整DNA序列是已知的。
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