• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

槲皮素通过抑制实验性近视中 PERK-EIF2α 轴减轻巩膜重塑。

Quercetin Alleviates Scleral Remodeling Through Inhibiting the PERK-EIF2α Axis in Experiment Myopia.

机构信息

Shandong University of Traditional Chinese Medicine, Jinan, China.

Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China.

出版信息

Invest Ophthalmol Vis Sci. 2024 Nov 4;65(13):11. doi: 10.1167/iovs.65.13.11.

DOI:10.1167/iovs.65.13.11
PMID:39504054
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11549929/
Abstract

PURPOSE

This study aims to investigate the effect of quercetin (QUE) on scleral remodeling by inhibiting the PERK-EIF2α signaling pathway and to evaluate its potential role in slowing myopia.

METHODS

Lens-induced myopia (LIM) guinea pigs were obtained and treated with QUE. After 4 and 6 weeks of treatments, ocular biological measurements were conducted. Hematoxylin and eosin (H&E) staining was used to observe the changes in scleral morphology and thickness, and Masson staining was used to examine scleral collagen fiber arrangement. Quantitative PCR (qPCR) and Western bolt were utilized to detect the mRNA and protein expression of PERK, EIF2α, MMP-2, TIMP-2, and collagen I in the scleral tissues. Calcium ion flow in each group was measured using noninvasive micro-test technology, and reactive oxygen species levels were detected by flow cytometry.

RESULTS

Compared with the LIM group, the ocular measurements showed that the refractive errors and axial length of the eyes were significantly reduced in the LIM + QUE group (P < 0.01). H&E and Masson staining showed that sclera in the LIM + QUE group was thickened, collagen was dense, and the fiber gap was reduced. In the LIM + QUE group, the expression levels of PERK, EIF2α, and MMP-2 were decreased, whereas the expression levels of TIMP-2 and collagen I were increased. Calcium release and reactive oxygen species (ROS) in the LIM + QUE group were decreased.

CONCLUSIONS

Quercetin ameliorates scleral remodeling in myopic guinea pigs by inhibiting the PERK-EIF2α signaling pathway, thereby alleviating the progression of myopia. These findings provide new experimental evidence for the potential application of quercetin in myopia prevention and treatment.

摘要

目的

本研究旨在通过抑制 PERK-EIF2α 信号通路来研究槲皮素（QUE）对巩膜重塑的影响，并评估其在减缓近视中的潜在作用。

方法

建立鸡眼球晶状体诱导型近视（LIM）模型，并用 QUE 进行处理。在治疗 4 周和 6 周后，进行眼部生物学测量。使用苏木精和伊红（H&E）染色观察巩膜形态和厚度的变化，使用 Masson 染色观察巩膜胶原纤维排列。利用实时定量聚合酶链反应（qPCR）和 Western blot 检测巩膜组织中 PERK、EIF2α、MMP-2、TIMP-2 和胶原 I 的 mRNA 和蛋白表达。利用非侵入性微测试技术测量各组钙离子流，通过流式细胞术检测活性氧（ROS）水平。

结果

与 LIM 组相比，LIM+QUE 组的眼屈光不正和眼轴明显减小（P＜0.01）。H&E 和 Masson 染色显示，LIM+QUE 组巩膜增厚，胶原致密，纤维间隙减小。在 LIM+QUE 组中，PERK、EIF2α 和 MMP-2 的表达水平降低，而 TIMP-2 和胶原 I 的表达水平升高。LIM+QUE 组钙离子释放和活性氧（ROS）减少。

结论

槲皮素通过抑制 PERK-EIF2α 信号通路改善近视豚鼠的巩膜重塑，从而减缓近视的进展。这些发现为槲皮素在近视预防和治疗中的潜在应用提供了新的实验依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/4ac3ee6fbb5a/iovs-65-13-11-f010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/5a5f2aa02917/iovs-65-13-11-f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/de0a3fe8548d/iovs-65-13-11-f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/7da1bca3fd04/iovs-65-13-11-f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/f913f6c7ef68/iovs-65-13-11-f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/dbc1a0be5327/iovs-65-13-11-f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/c9088a5dbacc/iovs-65-13-11-f006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/45d456038fec/iovs-65-13-11-f007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/6fcbc4dae7f8/iovs-65-13-11-f008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/4fa1cf95aa22/iovs-65-13-11-f009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/4ac3ee6fbb5a/iovs-65-13-11-f010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/5a5f2aa02917/iovs-65-13-11-f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/de0a3fe8548d/iovs-65-13-11-f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/7da1bca3fd04/iovs-65-13-11-f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/f913f6c7ef68/iovs-65-13-11-f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/dbc1a0be5327/iovs-65-13-11-f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/c9088a5dbacc/iovs-65-13-11-f006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/45d456038fec/iovs-65-13-11-f007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/6fcbc4dae7f8/iovs-65-13-11-f008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/4fa1cf95aa22/iovs-65-13-11-f009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b55/11549929/4ac3ee6fbb5a/iovs-65-13-11-f010.jpg

相似文献

1
Quercetin Alleviates Scleral Remodeling Through Inhibiting the PERK-EIF2α Axis in Experiment Myopia.槲皮素通过抑制实验性近视中 PERK-EIF2α 轴减轻巩膜重塑。
Invest Ophthalmol Vis Sci. 2024 Nov 4;65(13):11. doi: 10.1167/iovs.65.13.11.
2
Effects of riboflavin/ultraviolet-A scleral collagen cross-linking on regional scleral thickness and expression of MMP-2 and MT1-MMP in myopic guinea pigs.核黄素/紫外线 A 巩膜胶原交联术对近视豚鼠局部巩膜厚度和 MMP-2、MT1-MMP 表达的影响。
PLoS One. 2023 Jan 18;18(1):e0279111. doi: 10.1371/journal.pone.0279111. eCollection 2023.
3
Scleral Remolding-Related Gene Expression After Scleral Collagen Cross-Linking Using Ultraviolet A and Riboflavin in Myopic Guinea Pig Model.在近视豚鼠模型中，使用紫外线A和核黄素进行巩膜胶原交联后与巩膜重塑相关的基因表达
Curr Eye Res. 2023 Apr;48(4):392-401. doi: 10.1080/02713683.2022.2156549. Epub 2022 Dec 15.
4
Upregulation of regulator of G-protein signaling 2 in the sclera of a form deprivation myopic animal model.形觉剥夺性近视动物模型巩膜中G蛋白信号调节因子2的上调
Mol Vis. 2014 Jul 2;20:977-87. eCollection 2014.
5
RNA sequencing analysis of long non-coding RNA expression in ocular posterior poles of guinea pig myopia models.豚鼠近视模型眼球后极区长链非编码 RNA 表达的 RNA 测序分析。
Mol Vis. 2020 Mar 5;26:117-134. eCollection 2020.
6
Altered TGF-β2 and bFGF expression in scleral desmocytes from an experimentally-induced myopia guinea pig model.实验性诱导近视豚鼠模型巩膜成纤维细胞中 TGF-β2 和 bFGF 的表达改变。
Graefes Arch Clin Exp Ophthalmol. 2013 Apr;251(4):1133-44. doi: 10.1007/s00417-013-2269-8. Epub 2013 Feb 5.
7
Scleral hypoxia is a target for myopia control.巩膜缺氧是近视控制的靶点。
Proc Natl Acad Sci U S A. 2018 Jul 24;115(30):E7091-E7100. doi: 10.1073/pnas.1721443115. Epub 2018 Jul 9.
8
The miR-15b-5p/miR-379-3p-FOXO axis regulates cell cycle and apoptosis in scleral remodeling during experimental myopia.miR-15b-5p/miR-379-3p-FOXO 轴调控实验性近视巩膜重塑过程中的细胞周期和细胞凋亡。
J Transl Med. 2024 Jul 30;22(1):710. doi: 10.1186/s12967-024-05523-x.
9
Scleral PERK and ATF6 as targets of myopic axial elongation of mouse eyes.巩膜 PERK 和 ATF6 作为小鼠眼球近视轴向伸长的靶点。
Nat Commun. 2022 Oct 10;13(1):5859. doi: 10.1038/s41467-022-33605-1.
10
The Role of the RhoA/ROCK Signaling Pathway in Mechanical Strain-Induced Scleral Myofibroblast Differentiation.RhoA/ROCK 信号通路在机械应变诱导巩膜成纤维细胞分化中的作用。
Invest Ophthalmol Vis Sci. 2018 Jul 2;59(8):3619-3629. doi: 10.1167/iovs.17-23580.

引用本文的文献

1
Quercetin-loaded exosomes delivery system prevents myopia progression by targeting endoplasmic reticulum stress and ferroptosis in scleral fibroblasts.载槲皮素外泌体递送系统通过靶向巩膜成纤维细胞内质网应激和铁死亡来预防近视进展。
Mater Today Bio. 2025 May 28;32:101896. doi: 10.1016/j.mtbio.2025.101896. eCollection 2025 Jun.
2
Distinct Transcriptomic Profiles of Cultured Anterior and Posterior Populations of Human Infant Scleral Fibroblasts: Including Dopamine Receptors.人类婴儿巩膜成纤维细胞前体和后体培养物的不同转录组特征：包括多巴胺受体
Invest Ophthalmol Vis Sci. 2025 May 1;66(5):29. doi: 10.1167/iovs.66.5.29.

本文引用的文献

1
The role of oxidative stress in the pathogenesis of ocular diseases: an overview.氧化应激在眼病发病机制中的作用：概述。
Mol Biol Rep. 2024 Mar 27;51(1):454. doi: 10.1007/s11033-024-09425-5.
2
Bisphenol A exposure triggers endoplasmic reticulum stress pathway leading to ocular axial elongation in mice.双酚A暴露引发内质网应激途径，导致小鼠眼轴伸长。
Front Med (Lausanne). 2023 Sep 7;10:1255121. doi: 10.3389/fmed.2023.1255121. eCollection 2023.
3
Global risk factor analysis of myopia onset in children: A systematic review and meta-analysis.
全球儿童近视发病风险因素分析：系统评价和荟萃分析。
PLoS One. 2023 Sep 20;18(9):e0291470. doi: 10.1371/journal.pone.0291470. eCollection 2023.
4
Intravitreal Short-Hairpin RNA Attenuated Adeno-Associated Virus-Induced Knockdown of Amphiregulin and Axial Elongation in Experimental Myopia.玻璃体腔短发夹 RNA 减弱腺相关病毒诱导的实验性近视中 Amphiregulin 的敲低和轴向伸长。
Invest Ophthalmol Vis Sci. 2023 Apr 3;64(4):11. doi: 10.1167/iovs.64.4.11.
5
Quercetin: Its Antioxidant Mechanism, Antibacterial Properties and Potential Application in Prevention and Control of Toxipathy.槲皮素：其抗氧化机制、抗菌特性及在防治中毒症中的潜在应用。
Molecules. 2022 Oct 3;27(19):6545. doi: 10.3390/molecules27196545.
6
Scleral PERK and ATF6 as targets of myopic axial elongation of mouse eyes.巩膜 PERK 和 ATF6 作为小鼠眼球近视轴向伸长的靶点。
Nat Commun. 2022 Oct 10;13(1):5859. doi: 10.1038/s41467-022-33605-1.
7
ROS and Endoplasmic Reticulum Stress in Pulmonary Disease.肺部疾病中的活性氧与内质网应激
Front Pharmacol. 2022 Apr 26;13:879204. doi: 10.3389/fphar.2022.879204. eCollection 2022.
8
The PERKs of mitochondria protection during stress: insights for PERK modulation in neurodegenerative and metabolic diseases.应激期间线粒体保护的益处：对神经退行性疾病和代谢性疾病中PERK调节的见解
Biol Rev Camb Philos Soc. 2022 Oct;97(5):1737-1748. doi: 10.1111/brv.12860. Epub 2022 Apr 26.
9
Quercetin: an effective polyphenol in alleviating diabetes and diabetic complications.槲皮素：一种有效缓解糖尿病及其并发症的多酚类物质。
Crit Rev Food Sci Nutr. 2023;63(28):9163-9186. doi: 10.1080/10408398.2022.2067825. Epub 2022 Apr 25.
10
Health Benefits of Quercetin in Age-Related Diseases.槲皮素在与年龄相关疾病中的健康益处。
Molecules. 2022 Apr 13;27(8):2498. doi: 10.3390/molecules27082498.