Nash T E, Aggarwal A
J Immunol. 1986 Apr 1;136(7):2628-32.
Previous studies showed that some Giardia lamblia isolates differ and can be categorized on the basis of their DNA banding patterns after digestion with endonuclease restriction enzymes, surface antigens, and excretory-secretory (E-S) products. In the present study, monoclonal antibodies (McAb) were produced that reacted with one specific group of Giardia isolates. These McAb recognized a 170,000 dalton antigen, which was present on the surface of these Giardia and released into the medium as an E-S antigen. This antigen was previously characterized and found to distinguish this subgroup of Giardia. In addition, these McAb were cytotoxic only for this subgroup of Giardia. Immobilization occurred immediately, and killing was documented by 7 min. The mechanism(s) of killing remains unknown but was shown to be complement independent and did not occur with Fab'. These McAb identifies certain isolates and can be used to type Giardia.
先前的研究表明，一些蓝氏贾第鞭毛虫分离株存在差异，可根据其经核酸内切限制酶消化后的DNA条带模式、表面抗原以及排泄分泌（E-S）产物进行分类。在本研究中，制备了与一组特定蓝氏贾第鞭毛虫分离株发生反应的单克隆抗体（McAb）。这些单克隆抗体识别一种170,000道尔顿的抗原，该抗原存在于这些蓝氏贾第鞭毛虫的表面，并作为E-S抗原释放到培养基中。该抗原先前已被鉴定，并发现可区分这一蓝氏贾第鞭毛虫亚组。此外，这些单克隆抗体仅对该蓝氏贾第鞭毛虫亚组具有细胞毒性。立即发生固定，7分钟后记录到杀伤情况。杀伤机制尚不清楚，但已表明其不依赖补体，且Fab'片段不会引发杀伤。这些单克隆抗体可识别某些分离株，可用于对蓝氏贾第鞭毛虫进行分型。
