Lacy M J, Voss E W
J Immunol Methods. 1986 Mar 13;87(2):169-77. doi: 10.1016/0022-1759(86)90527-2.
Immunized BALB/c mice were injected with 10(6) viable Sp2/0-Ag14 cells to induce ascites fluid concurrently with maximum serum antibody activity. Ascites fluids developed in a systematic manner, yielded large amounts of specific polyclonal antibody even when the mice were injected with relatively small amounts of immunogen. A combination of an initial pristane injection prior to 3 intraperitoneal immunizations of immunogen emulsified in Freund's complete adjuvant (total volume of 0.1-0.2 ml per injection), followed 4 days later by injection of 10(6) viable Sp2/0-Ag14 cells, resulted in a consistent production of ascites fluid with substantial specific antibody activity.
给免疫的BALB/c小鼠注射10⁶个活的Sp2/0-Ag14细胞以诱导腹水，同时产生最大血清抗体活性。腹水以一种系统的方式形成，即使给小鼠注射相对少量的免疫原，也能产生大量特异性多克隆抗体。在腹腔内用弗氏完全佐剂乳化的免疫原进行3次免疫之前，先注射一次 pristane（总量为每次注射0.1 - 0.2 ml），4天后再注射10⁶个活的Sp2/0-Ag14细胞，这种组合可导致持续产生具有大量特异性抗体活性的腹水。
