Bortoluzzi C, Segura-Wang M, Aureli R, Leduc A, Iuspa M A, Cowieson A J
dsm-firmenich, Kaiseraugst, Switzerland.
dsm-firmenich, Animal Nutrition and Health R&D Center, Tulln, Austria.
Poult Sci. 2024 Dec;103(12):104451. doi: 10.1016/j.psj.2024.104451. Epub 2024 Oct 29.
The objective of the present study was to evaluate the effect of a precision biotic (PB; Symphiome®, dsm-firmenich) on the growth performance, nutrient digestibility, litter quality, blood uric acid (UA) concentration, cecal short-chain fatty acids (SCFA) concentration, and ileal and cecal microbiome of broiler chickens fed corn or wheat-based diets. One-day-old broiler chicks were placed in a completely randomized design (2 × 2 factorial scheme) in 4 treatments, 8 replicates/treatment, and 16 birds/replicate. The treatments were a corn or wheat and soybean meal-based diet without or with supplementation of 0.9 kg/MT of PB. All the birds were vaccinated against coccidiosis at the time of placement (Paracox 5). Growth performance was evaluated weekly and at the end of the experiment. At d 35, blood was collected to determine UA concentration and cecal content for SCFA concentration analysis. Ileal digesta was also collected for nutrient digestibility analysis, and ileal digesta and cecal content were collected for microbiome analysis. The data were submitted to two-way ANOVA (P ≤ 0.05), and LSM was used to separate the means in case of interaction. During the starter and grower phases of the study, a better body weight gain (P = 0.0008 and P = 0.04, respectively) was observed in birds fed wheat compared to corn-based diets. From 28 to 36 d of age, the supplementation of PB increased feed intake (P = 0.05), and tended (P = 0.06) to improve the feed conversion ratio by 4.3 points vs non-supplementated birds. Birds fed wheat-based diets had higher (P = 0.02) blood UA than corn based-diets fed birds, and the supplementation of PB led to a reduction (P = 0.02) of blood UA compared to non-supplemented birds. The supplementation of PB changed the abundance of core metabolic pathways of the microbiome, mostly related to protein metabolism, which led to a reduction in blood UA concentration and increase of cecal SCFA concentrations. In conclusion, by beneficially modulating the microbiome, the supplementation of PB was translated into improved growth performance of broiler chickens fed corn or wheat-based diets.
本研究的目的是评估一种精准生物制剂（PB；Symphiome®，帝斯曼-芬美意公司）对以玉米或小麦为基础日粮的肉鸡生长性能、养分消化率、垫料质量、血尿酸（UA）浓度、盲肠短链脂肪酸（SCFA）浓度以及回肠和盲肠微生物群的影响。1日龄的肉鸡雏鸡采用完全随机设计（2×2析因方案），分为4个处理组，每个处理组8个重复，每个重复16只鸡。处理组分别为以玉米或小麦和豆粕为基础的日粮，不添加或添加0.9 kg/MT的PB。所有鸡在入舍时均接种球虫病疫苗（Paracox 5）。每周及实验结束时评估生长性能。在第35天，采集血液以测定UA浓度，并采集盲肠内容物用于SCFA浓度分析。还采集回肠食糜用于养分消化率分析，并采集回肠食糜和盲肠内容物用于微生物群分析。数据采用双向方差分析（P≤0.05），如有交互作用，则用最小二乘均值法分离均值。在研究的育雏期和生长育肥期，与以玉米为基础日粮的鸡相比，以小麦为基础日粮的鸡体重增加更好（分别为P = 0.0008和P = 0.04）。从28日龄到36日龄，添加PB增加了采食量（P = 0.05），与未添加PB的鸡相比，有提高饲料转化率4.3个百分点的趋势（P = 0.06）。以小麦为基础日粮的鸡的血尿酸水平高于以玉米为基础日粮的鸡（P = 0.02），与未添加PB的鸡相比，添加PB可降低血尿酸水平（P = 0.02）。添加PB改变了微生物群核心代谢途径的丰度，主要与蛋白质代谢有关，这导致血尿酸浓度降低和盲肠SCFA浓度升高。总之，通过有益地调节微生物群，添加PB可转化为以玉米或小麦为基础日粮的肉鸡生长性能的改善。
