Shandong New Hope Liuhe Group, Qingdao, Shandong 266000, PR China.
DSM Nutritional Products, Shanghai 201203, PR China.
Poult Sci. 2023 May;102(5):102596. doi: 10.1016/j.psj.2023.102596. Epub 2023 Feb 16.
Precision biotics (PBs) are chemically synthesized complex glycans that modulate specific microbiome metabolic functions. The objective of the present study was to evaluate the effect of the supplementation of PB on the growth performance, and cecal microbiome modulation of broiler chickens raised under commercial conditions. A total of 190,000-day-old Ross 308 straight-run broilers were randomly assigned to 2 dietary treatments. There were 5 houses per treatment with 19,000 birds per house. In each house, there were 6 rows of battery cages with 3 tiers. The 2 dietary treatments included a control diet (a commercial broiler diet) and a PB supplemented diet at 0.9 kg/MT. On a weekly basis, 380 birds were randomly selected for body weight (BW) determination. At 42 d of age, the BW and feed intake (FI) of each house were recorded, the feed conversion ratio (cFCR) was calculated and corrected with the final BW, and the European production index (EPI) was calculated. Additionally, 8 birds per house (40 birds/experimental group) were randomly selected to collect cecal content for microbiome analysis. The supplementation of PB significantly improved (P < 0.05) the BW of the birds at 7, 14, and 21 d and numerically improved the BW of the birds by 64 and 70 g at 28 and 35 d of age, respectively. At 42 d, the PB numerically improved BW by 52 g, and significantly improved (P < 0.05) the cFCR by 2.2 points and the EPI by 13 points. The functional profile analysis showed a clear and significant difference in the cecal microbiome metabolism between control vs. PB supplemented birds. A higher abundance of pathways was modulated by PB which were associated with amino acid fermentation and putrefaction, particularly from lysine, arginine, proline, histidine, and tryptophane which led to a significant increase (P = 0.0025) in the Microbiome Protein Metabolism Index (MPMI) compared to nonsupplemented birds. In conclusion, the supplementation of PB efficiently modulated pathways related to protein fermentation and putrefaction, resulting in higher MPMI and improved growth performance of broilers.
精准益生菌（PBs）是经过化学合成的复杂糖，可调节特定微生物组的代谢功能。本研究旨在评估 PB 补充对商业条件下饲养的肉鸡生长性能和盲肠微生物组调节的影响。总共 19 万日龄罗斯 308 直线型肉鸡被随机分配到 2 种饮食处理中。每个处理有 5 个鸡舍，每个鸡舍有 19000 只鸡。在每个鸡舍中，有 6 排笼，有 3 层。2 种饮食处理包括对照饮食（商业肉鸡饮食）和补充 0.9 千克/吨 PB 的饮食。每周随机选择 380 只鸡进行体重（BW）测定。在 42 日龄时，记录每个鸡舍的 BW 和采食量（FI），用最终 BW 校正饲料转化率（cFCR），并计算欧洲生产指数（EPI）。此外，每个鸡舍随机选择 8 只鸡（每个实验组 40 只鸡）收集盲肠内容物进行微生物组分析。PB 的补充显著提高了（P<0.05）7、14 和 21 日龄鸡的 BW，分别使 28 和 35 日龄鸡的 BW 增加了 64 和 70 克。42 日龄时，PB 使 BW 增加了 52 克，显著降低（P<0.05）cFCR 2.2 点，EPI 增加 13 点。功能谱分析显示，对照组和 PB 补充组盲肠微生物组代谢存在明显且显著的差异。PB 调节了更多与氨基酸发酵和腐败相关的途径，特别是赖氨酸、精氨酸、脯氨酸、组氨酸和色氨酸，这导致微生物组蛋白质代谢指数（MPMI）显著增加（P=0.0025）与未补充组相比。总之，PB 的补充有效地调节了与蛋白质发酵和腐败相关的途径，导致更高的 MPMI 和肉鸡生长性能的提高。
