Yang Xiupei, Xie Xiaoyi, Jiang Ling, Fan Yuxiu, Zhang Chenglin, Wang Ya
College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong, 637000, China.
College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong, 637000, China.
Talanta. 2025 Feb 1;283:127131. doi: 10.1016/j.talanta.2024.127131. Epub 2024 Nov 2.
Most conventional nanozymes have poor specificity and low activity, and designing high-performance nanozymes remains a challenge. In contrast, single-atom nanozymes have high atom utilization and high reactivity. Here, we prepared Cu single-atom nanozymes (Cu-NC) with excellent peroxidase-like activity by high-temperature pyrolysis using Cu as a transition metal source. The introduction of Cu formed the Cu-N active site, which accelerated charge transfer between the reactants and the active site and was the key for improving the activity. With Cu-NC as a catalyst, HO rapidly oxidized 3,3',5,5'-tetramethylbenzidine (TMB) to oxTMB, and the solution turned blue with strong absorption at 652 nm. Because d-penicillamine (D-PA) can reduce oxTMB or react with reactive oxygen species radicals to inhibit the color reaction, we built a colorimetric sensing platform around Cu-NC for the determination of D-PA and successfully used it for the determination of D-PA in urine samples. This work provides new ideas for the design of high-performance nanozymes and the detection of D-PA in real environments.
大多数传统纳米酶具有较差的特异性和较低的活性，设计高性能纳米酶仍然是一个挑战。相比之下，单原子纳米酶具有高原子利用率和高反应活性。在此，我们以铜作为过渡金属源，通过高温热解制备了具有优异类过氧化物酶活性的铜单原子纳米酶（Cu-NC）。铜的引入形成了Cu-N活性位点，加速了反应物与活性位点之间的电荷转移，这是提高活性的关键。以Cu-NC作为催化剂，HO迅速将3,3',5,5'-四甲基联苯胺（TMB）氧化为oxTMB，溶液变蓝并在652 nm处有强烈吸收。由于d-青霉胺（D-PA）可以还原oxTMB或与活性氧自由基反应以抑制显色反应，我们围绕Cu-NC构建了一个比色传感平台用于测定D-PA，并成功将其用于尿液样本中D-PA的测定。这项工作为高性能纳米酶的设计以及实际环境中D-PA的检测提供了新思路。
