Department of Orthopedics, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215300, China; Department of Orthopedics, Kunshan Hospital of Chinese Medicine, Kunshan, Jiangsu 215300, China.
Department of Orthopedics, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215300, China; Department of Orthopedics, The Affiliated Suqian Hospital of Xuzhou Medical University, Suqian, Jiangsu 223800, China.
Bone. 2025 Jan;190:117315. doi: 10.1016/j.bone.2024.117315. Epub 2024 Nov 4.
Tissue engineering employs the use of bioactive materials to facilitate the filling and acceleration of bone defect healing, thereby introducing novel concepts to the field of in situ bone repair. Some studies have shown that periosteum plays an important role in bone regeneration and repair. In this study, biomimetic periosteum-bone scaffolds were prepared by depositing poly-L-lactic acid (PLLA) electrospun fibers on the surface of the gelatin/chitosan cryogel to mimic the bone and periosteum structure, respectively. To improve the bioactivity of the scaffold, bone morphogenetic protein-2 (BMP-2) was loaded into a loose porous mesh-like cryogel, while platelet-derived growth factor-BB (PDGF-BB) was encapsulated in the core of PLLA nanofibers with core-shell structure. Both of these two growth factors were released locally at the site of bone defect, where they exert a synergistic effect on osteogenesis, thereby greatly accelerating bone healing. The in vitro experiments demonstrated that the biomimetic periosteum-bone scaffolds exhibited favourable biocompatibility and osteogenesis ability. Furthermore, the in vivo experiments indicated that the composite scaffold repaired rat skull defects in a more rapid and effective manner. In conclusion, biomimetic periosteum-bone scaffolds with codelivery of BMP-2 and PDGF-BB shows significant potential for bone regeneration.
组织工程利用生物活性材料来促进骨缺损的填充和加速愈合，从而为原位骨修复领域带来新的概念。一些研究表明，骨膜在骨再生和修复中起着重要作用。在这项研究中，通过在明胶/壳聚糖冷冻凝胶表面沉积聚 L-乳酸（PLLA）电纺纤维，分别模拟骨和骨膜结构，制备了仿生骨膜-骨支架。为了提高支架的生物活性，将骨形态发生蛋白-2（BMP-2）负载到疏松多孔的网状冷冻凝胶中，而血小板衍生生长因子-BB（PDGF-BB）则被包裹在具有核壳结构的 PLLA 纳米纤维的核心中。这两种生长因子都在骨缺损部位局部释放，对成骨具有协同作用，从而大大加速了骨愈合。体外实验表明，仿生骨膜-骨支架具有良好的生物相容性和成骨能力。此外，体内实验表明，复合支架以更快速和有效的方式修复了大鼠颅骨缺损。总之，具有 BMP-2 和 PDGF-BB 共递送的仿生骨膜-骨支架在骨再生方面具有显著的潜力。
