Department of Orthopaedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, People's Republic of China. Department of Hand Surgery, Ningbo City Sixth Hospital, Ningbo 315040, People's Republic of China.
Biomed Mater. 2018 Jan 9;13(2):025001. doi: 10.1088/1748-605X/aa93c0.
The aim of this study was to prepare chitosan-collagen (CS/COL) scaffolds that could release fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein 2 (BMP-2), and to study the effect of this scaffold on bone repair. By improving the double emulsion/solvent evaporation technique, BMP-2 was encapsulated in poly(lactic acid)-poly(ethylene glycol)-poly(lactic acid) (PELA) microcapsules, to the surface of which FGF-2 was attached. The CS/COL scaffold carrying the microcapsules was prepared by freeze-drying. Periosteum derived cells (PDCs) were extracted and cultured on the scaffolds to study their proliferation and differentiation on the scaffolds. In addition, the effects of the scaffolds were investigated on rats with skull defects by micro-computed tomography and histology. We successfully prepared PELA microcapsules with external adherence to FGF-2 and encapsulated with BMP-2. The CS/COL scaffolds were porous and PDCs adhered, proliferated and underwent osteogenic differentiation on the scaffolds. The sequential release of FGF-2/BMP-2 had better osteogenic efficacy than other groups. Our results suggest that CS/COL scaffolds that bind FGF-2 and BMP-2 in combination with PDCs could be a promising new strategy for tissue engineering periosteum.
本研究旨在制备壳聚糖-胶原蛋白（CS/COL）支架，使其能够释放成纤维细胞生长因子-2（FGF-2）和骨形态发生蛋白 2（BMP-2），并研究该支架对骨修复的影响。通过改进双重乳液/溶剂蒸发技术，将 BMP-2 包封在聚乳酸-聚乙二醇-聚乳酸（PELA）微胶囊中，并将 FGF-2 附着在微胶囊的表面。通过冷冻干燥制备载有微胶囊的 CS/COL 支架。提取和培养骨膜来源细胞（PDCs）在支架上，研究其在支架上的增殖和分化。此外，通过微计算机断层扫描和组织学研究了支架对颅骨缺损大鼠的作用。我们成功制备了具有外部附着 FGF-2 和封装 BMP-2 的 PELA 微胶囊。CS/COL 支架是多孔的，PDCs 附着、增殖并在支架上进行成骨分化。FGF-2/BMP-2 的顺序释放具有更好的成骨效果。我们的结果表明，结合 PDCs 绑定 FGF-2 和 BMP-2 的 CS/COL 支架可能是组织工程骨膜的一种有前途的新策略。
