Chen Xu, Xiao Naiyu, Xiang Hongxia, Li Shuyi, Zhu Zhenzhou, Cong Xin, Chen Xiang, Cheng Shuiyuan
School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China; National R&D center for Se-rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan, 430023, China.
School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China; National R&D center for Se-rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan, 430023, China.
Int J Biol Macromol. 2024 Dec;282(Pt 4):137211. doi: 10.1016/j.ijbiomac.2024.137211. Epub 2024 Nov 4.
This study involved the fabrication of double-layer intelligent films using chitosan (CS), polyvinyl alcohol (PVA), grape skin anthocyanin (GSA), gellan gum (GG) and selenium nanoparticles (SeNPs). The CS/PVA/GSA layer functioned as the internal indicator layer, and the GG/SeNPs layer acted as the external layer for antioxidant and antimicrobial purposes. SEM, FTIR, XRD, and TGA results confirmed the successful fabrication of double-layer films as well as the presence of hydrogen bonding interaction between the two layers. The tensile strength of double-layer films (8.06 MPa-9.61 MPa) fallen between that of single-layer CS/PVA/GSA film (12.51 MPa) and GG/SeNPs film (1.50 MPa-7.67 MPa). The double-layer films demonstrated good UV-blocking abilities, as well as outstanding antioxidant (ABTS scavenging rate can be up to ∼80 %) and antimicrobial properties. Compared with single-layer CS/PVA/GSA film, the double-layer film incorporated with 6.6 wt% SeNPs (CPG/GS2 film) possessed a more rapid and stronger response towards NH/acetic acid as well as enhanced storage stability. Furthermore, the CPG/GS2 film can increase the shelf life of strawberries at 25 °C by 4 days, and its visible color change showed strong correlation with the weight loss rate (R = 0.99) and hardness (R = 0.98) of strawberries.
本研究涉及使用壳聚糖（CS）、聚乙烯醇（PVA）、葡萄皮花青素（GSA）、结冷胶（GG）和硒纳米颗粒（SeNPs）制备双层智能薄膜。CS/PVA/GSA层作为内部指示层，而GG/SeNPs层作为用于抗氧化和抗菌目的的外层。扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）和热重分析（TGA）结果证实了双层薄膜的成功制备以及两层之间存在氢键相互作用。双层薄膜的拉伸强度（8.06MPa - 9.61MPa）介于单层CS/PVA/GSA薄膜（12.51MPa）和GG/SeNPs薄膜（1.50MPa - 7.67MPa）之间。双层薄膜表现出良好的紫外线阻隔能力，以及出色的抗氧化性能（ABTS清除率可达约80%）和抗菌性能。与单层CS/PVA/GSA薄膜相比，掺入6.6wt% SeNPs的双层薄膜（CPG/GS2薄膜）对NH/乙酸具有更快、更强的响应以及增强的储存稳定性。此外，CPG/GS2薄膜可使草莓在25°C下的货架期延长4天，其可见颜色变化与草莓的失重率（R = 0.99）和硬度（R = 0.98）显示出很强的相关性。
