Wu Liujie, Lai Liuying, Wu Weijun, Wang Yongzhuang, Mo Ganhui, Kobayashi Yuriko, Ogo Naohisa, Koyama Hiroyuki
School of Environmental and Life Science, Nanning Normal University, Nanning, China.
School of Agricultural Engineering, Guangxi Vocational and Technical College, Nanning, China.
J Plant Res. 2025 Jan;138(1):121-129. doi: 10.1007/s10265-024-01594-5. Epub 2024 Nov 6.
Chemical genetics is a multidisciplinary research method. In this study, it is used to screen compounds that promote aluminum-induced malate secretion in Arabidopsis thaliana. Inhibition of p38 mitogen-activated protein kinase (p38 MAPK; LY2228820) significantly increased the transcription of Arabidopsis thaliana aluminum-activated malate transporter 1 (AtALMT1) and sensitive to proton rhizotoxicity 1 (STOP1)-regulated genes, multidrug and toxic compound extrusion and aluminum sensitive 3, but not AtSTOP1 and the Al-biomarker genes At3g28510, At5g13320, suggesting that LY2228820 increased the early expression of STOP1-regulated genes without affecting AtSTOP1 expression. Inhibition of p38 MAPK (LY2228820) and Aurora A (MLN8237) increased aluminum-activated malate transport via AtALMT1, suggesting that both MLN8237 and LY2228820 interfere with AtALMT1 activity. An increase in root elongation was also observed in Arabidopsis after applying compounds LY2228820 and MLN8237. Thus, both LY2228820 and MLN8237 may play important roles in alleviating the inhibitory effects of aluminum on roots.
化学遗传学是一种多学科研究方法。在本研究中，它被用于筛选促进拟南芥中铝诱导苹果酸分泌的化合物。抑制p38丝裂原活化蛋白激酶（p38 MAPK；LY2228820）显著增加了拟南芥铝激活苹果酸转运蛋白1（AtALMT1）以及对质子根毒性敏感1（STOP1）调控基因、多药和有毒化合物外排蛋白以及铝敏感3的转录，但不影响AtSTOP1以及铝生物标志物基因At3g28510、At5g13320的转录，这表明LY2228820增加了STOP1调控基因的早期表达，而不影响AtSTOP1的表达。抑制p38 MAPK（LY2228820）和极光激酶A（MLN8237）通过AtALMT1增加了铝激活的苹果酸转运，这表明MLN8237和LY2228820均干扰AtALMT1的活性。在施加化合物LY2228820和MLN8237后，拟南芥的根伸长也有所增加。因此，LY2228820和MLN8237在减轻铝对根的抑制作用中可能都起着重要作用。
