Li Changming, Tan Yong, Liu Chunyu, Gao Xun, Lv Zhong
School of Physics, Changchun University of Science and Technology, Changchun, 130022, China.
Engineering Tech. R&D Center Changchun Guanghua College, Changchun, 130033, China.
Sci Rep. 2024 Nov 7;14(1):27030. doi: 10.1038/s41598-024-77492-6.
The substitution of microbial fertilizer for chemical fertilizer can not only improve soil fertility but also effectively enhance rice quality. To investigate the effect of different amounts of combined application of chemical fertilizer and microbial fertilizer on the amylose content of rice, this study adopts theoretical calculations to compare the preprocessed Raman spectroscopy information of rice with reduced fertilization and establishes a recognition model for the amylose content of rice, which is used to detect the amylose content in rice. Based on the amylose spectral values measured by Raman spectroscopy and the known starch structure and functional groups, the Raman peaks are mainly distributed in the range of 400 cm to 1400 cm. The Raman characteristic peaks at 483 cm, 869 cm, 933 cm, 1082 cm, 1126 cm, 1335 cm, 1385 cm, and 1455 cm exhibit strong vibration modes, which are consistent with its main nutrient component of amylose. By comparing the measured amylose content in the regions treated with microbial fertilizer combined with different amounts of reduced fertilization and the spectral intensity values of amylose measured by Raman spectroscopy, the results show that the treatment of combining conventional amounts of microbial fertilizer with different amounts of reduced chemical fertilizer exhibits a decreasing trend in the amylose content of rice.
用微生物肥料替代化肥不仅可以提高土壤肥力，还能有效提升水稻品质。为研究不同用量的化肥与微生物肥料配施对水稻直链淀粉含量的影响，本研究采用理论计算方法，对比减施化肥处理下水稻的预处理拉曼光谱信息，建立水稻直链淀粉含量识别模型，用于检测水稻中的直链淀粉含量。基于拉曼光谱测定的直链淀粉光谱值以及已知的淀粉结构和官能团，拉曼峰主要分布在400cm至1400cm范围内。在483cm、869cm、933cm、1082cm、1126cm、1335cm、1385cm和1455cm处的拉曼特征峰呈现出强烈的振动模式，这与其直链淀粉的主要营养成分一致。通过比较微生物肥料与不同用量减施化肥配施区域实测的直链淀粉含量以及拉曼光谱测定的直链淀粉光谱强度值，结果表明，常规用量微生物肥料与不同用量减施化肥配施处理下，水稻直链淀粉含量呈下降趋势。
