Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Faisalabad, 38000, Pakistan.
School of Agriculture and Food Sustainability, The University of Queensland, Brisbane, 4072, Australia.
BMC Genomics. 2024 Nov 6;25(1):1046. doi: 10.1186/s12864-024-10958-z.
Genetic profiling of the biodiversity in cultivated crop plants is necessary to preserve important genes and utilize them in a breeding program. Cucumber is used as a model plant to study various characteristics of Cucurbitaceae. Its adaptation to a wide range of climatic conditions suggested analyzing the landraces. The present study was conducted to evaluate the differences, at the genetic level, among landraces spanning five continents. DNA extracted from fifty-six landraces selected from USDA germplasm bank to cover a global representative sample of world cucumber landraces was used for polymerase chain reaction using twenty-eight polymorphic expressed sequence tags simple sequence repeat (EST-SSR) markers. Twenty-eight EST-SSR markers covering all seven chromosomes yielded 98 bands with an average of 3.42 bands per marker. Polymorphic information content ranged from 0.00 (EC35) to 0.74 (EC17) with an average of 0.34. Six clusters provided an appropriate summary of the variation among the landraces, with the two largest groups including 32 (Asiatic) and 17 (European and American) landraces, respectively. Four small groups, three with two members, and one with one member (PI 525155-Egypt) were dissimilar to the two main groups. Landraces from the same region were often clustered together. Genetic similarity of the landraces was revealed by marker banding patterns. The locations of genetic diversity for cucumber landraces can be identified from this study.
对栽培作物的生物多样性进行基因谱分析对于保护重要基因并将其应用于育种计划是必要的。黄瓜被用作研究葫芦科各种特性的模式植物。它对广泛气候条件的适应表明需要分析地方品种。本研究旨在评估跨越五大洲的地方品种在遗传水平上的差异。从美国农业部种质库中选择的 56 个地方品种提取的 DNA 用于聚合酶链反应，使用 28 个多态性表达序列标签简单重复（EST-SSR）标记。28 个覆盖所有 7 条染色体的 EST-SSR 标记产生了 98 个带，平均每个标记产生 3.42 个带。多态信息含量范围从 0.00（EC35）到 0.74（EC17），平均值为 0.34。6 个聚类为地方品种之间的变异提供了适当的总结，其中两个最大的聚类分别包括 32 个（亚洲）和 17 个（欧洲和美洲）地方品种。四个小聚类，三个有两个成员，一个有一个成员（PI 525155-埃及）与两个主要聚类不同。来自同一地区的地方品种通常聚集在一起。标记带型揭示了地方品种的遗传相似性。本研究可以确定黄瓜地方品种遗传多样性的位置。
