Yan Xi, Shan Shengzhou, Li Xiaohui, Xu Qingshan, Yan Xiaojun, Ruan Roger, Cheng Pengfei
College of Food Science and Engineering, Ningbo University, Ningbo, Zhejiang, China.
Lijiang Cheng Hai Bao Er Biological Development Co., Ltd., Lijiang, Yunnan, China.
Front Microbiol. 2024 Oct 23;15:1436264. doi: 10.3389/fmicb.2024.1436264. eCollection 2024.
There has been an emergence of a diversity of microalgal mixotrophic synergistic mechanisms due to substrate differences. In this study, the effects of the mixotrophic culture of were examined. The maximum values of cell density, specific growth rate, and cell dry weight of were 3.52*10 cells/mL, 0.75 d, and 3.48 g/L in the mixotrophic mode, respectively. These were higher than the corresponding values of photoautotrophic or heterotrophic modes. Moreover, it was found that the concentrations of sodium bicarbonate consumed by the under mixotrophic and photoautotrophic modes were 635 mg/L/d and 505 mg/L/d, respectively; the concentrations of sodium acetate consumed by the under mixotrophic and heterotrophic modes were 614 mg/L/d and 645 mg/L/d, respectively. The activity of Rubisco was 9.36 U/mL in the mixotrophic culture, which was 3.09 and 4.85 times higher than that of the photoautotrophic and heterotrophic modes, respectively. This indicated that the differences for the carbon source absorption efficiency of in the mixotrophy led to different internal metabolic efficiencies when compared to photoautotroph or heterotrophy. Additionally, exhibits a more rapid energy metabolism efficiency when operating in the mixotrophic mode.
由于底物差异，出现了多种微藻混合营养协同机制。在本研究中，考察了[具体微藻名称未给出]混合营养培养的效果。在混合营养模式下，[具体微藻名称未给出]的细胞密度、比生长速率和细胞干重的最大值分别为3.52×10个细胞/毫升、0.75天和3.48克/升。这些值高于光合自养或异养模式下的相应值。此外，发现[具体微藻名称未给出]在混合营养和光合自养模式下消耗的碳酸氢钠浓度分别为635毫克/升/天和505毫克/升/天；[具体微藻名称未给出]在混合营养和异养模式下消耗的醋酸钠浓度分别为614毫克/升/天和645毫克/升/天。在混合营养培养中，核酮糖-1,5-二磷酸羧化酶（Rubisco）的活性为9.36单位/毫升，分别比光合自养和异养模式高3.09倍和4.85倍。这表明与光合自养或异养相比，[具体微藻名称未给出]在混合营养中对碳源吸收效率的差异导致了不同的内部代谢效率。此外，[具体微藻名称未给出]在混合营养模式下运行时表现出更快的能量代谢效率。
